| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Acetyl-Tubulin alpha-1A chain-Lys352 (311-360 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TUBA1A |
| Gene ID: | 7846 |
| Uniprot ID: | TBA1A_HUMAN |
| Immunogen Region: | 311-360 aa |
| Specificity: | Acetyl-Tubulin Alpha (K352) Polyclonal Antibody detects endogenous levels of Tubulin Alpha protein only when acetylated at K352. |
| Immunogen: | The antiserum was produced against synthesized Acetyl-peptide derived from the human TUBA1B around the Acetylation site of Lys352 at the amino acid range 311-360 |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution. Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (MATCAP1/KIAA0895L, VASH1 or VASH2) and tubulin tyrosine ligase (TTL), respectively. Tubulin alpha-1A chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1. Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules. In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport. Detyrosinated tubulin alpha-1A chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator. Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Protein Name | Tubulin Alpha-1a Chain Alpha-Tubulin 3 Tubulin B-Alpha-1 Tubulin Alpha-3 Chain Cleaved Into - Detyrosinated Tubulin Alpha-1a Chain |
| Database Links | Reactome: R-HSA-1445148 Reactome: R-HSA-190840 Reactome: R-HSA-190861 Reactome: R-HSA-2132295 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2565942 Reactome: R-HSA-3371497 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-389957 Reactome: R-HSA-389960 Reactome: R-HSA-389977 Reactome: R-HSA-437239 Reactome: R-HSA-5610787 Reactome: R-HSA-5617833 Reactome: R-HSA-5620912 Reactome: R-HSA-5620924 Reactome: R-HSA-5626467 Reactome: R-HSA-5663220 Reactome: R-HSA-6807878 Reactome: R-HSA-6811434 Reactome: R-HSA-6811436 Reactome: R-HSA-68877 Reactome: R-HSA-8852276 Reactome: R-HSA-8854518 Reactome: R-HSA-8955332 Reactome: R-HSA-9609690 Reactome: R-HSA-9609736 Reactome: R-HSA-9619483 Reactome: R-HSA-9646399 Reactome: R-HSA-9648025 Reactome: R-HSA-9668328 Reactome: R-HSA-983189 Reactome: R-HSA-9833482 |
| Cellular Localisation | Cytoplasm Cytoskeleton Flagellum Axoneme |
| Alternative Antibody Names | Anti-Tubulin Alpha-1a Chain antibody Anti-Alpha-Tubulin 3 antibody Anti-Tubulin B-Alpha-1 antibody Anti-Tubulin Alpha-3 Chain Cleaved Into - Detyrosinated Tubulin Alpha-1a Chain antibody Anti-TUBA1A antibody Anti-TUBA3 antibody |
Information sourced from Uniprot.org