• Western blot analysis of extracts of HeLa cells, using 53BP1 antibody (STJ11103538) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using 53BP1 rabbit monoclonal antibody (STJ11103538) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using 53BP1 rabbit monoclonal antibody (STJ11103538) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using 53BP1 rabbit monoclonal antibody (STJ11103538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-53BP1 antibody (STJ11103538)

Current Stock:
Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse
Short Description: Rabbit monoclonal antibody anti-53BP1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Monoclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: TP53BP1
Gene ID: 7158
Uniprot ID: TP53B_HUMAN
Immunogen: A synthesized peptide derived from human 53BP1
Post Translational Modifications Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Phosphorylated at basal level in the absence of DNA damage. Phosphorylated by ATM in response to DNA damage: phosphorylation at different sites promotes interaction with different set of proteins: phosphorylation at the N-terminus by ATM (residues from 6-178) promotes interaction with PAXIP1 and non-homologous end joining (NHEJ) of dysfunctional telomeres. Phosphorylation by ATM at residues that are located more C-terminus (residues 300-650) leads to promote interaction with RIF1. Interaction with RIF1 leads to disrupt interaction with NUDT16L1/TIRR. Phosphorylation at Thr-1609 and Ser-1618 in the UDR motif blocks interaction with H2AK15ub. Dephosphorylated by PPP4C. Hyperphosphorylation during mitosis correlates with its exclusion from chromatin and DNA lesions. Hyperphosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Dephosphorylated by PPP5C.
Function Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Protein Name Tp53-Binding Protein 1
P53-Binding Protein 1
Database Links Reactome: R-HSA-3232118
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Localizes To The Nucleus In Absence Of Dna Damage
Following Dna Damage
Recruited To Sites Of Dna Damage
Such As Double Stand Breaks (Dsbs): Recognizes And Binds Histone H2a Monoubiquitinated At 'Lys-15' (H2ak15ub) And Histone H4 Dimethylated At 'Lys-20' (H4k20me2)
Two Histone Marks That Are Present At Dsbs Sites
Associated With Kinetochores During Mitosis
Alternative Antibody Names Anti-Tp53-Binding Protein 1 antibody
Anti-53bp1 antibody
Anti-P53-Binding Protein 1 antibody
Anti-P53bp1 antibody
Anti-TP53BP1 antibody

Information sourced from Uniprot.org

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