Signal Transduction | Research Area | St John's Laboratory

Immunofluorescence analysis of HeLa cells using Smad5 Polyclonal Antibody (STJ115958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Smad5 antibody (200-300) (STJ115958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g Smad5 antibody (STJ11101654). Western blot was performed from the immunoprecipitate using Smad5 antibody (STJ11101654) at a dilution of 1:1000.

Anti-SMAD5 antibody (200-300) [S4MR] (STJ11101654)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SMAD5 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-SMAD5 antibody (244-262 aa) [R07-2A5] (STJA0011975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£264.50

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Western blot analysis using Smad5 monoclonal antibody against mouse testis lysate.

Anti-SMAD5 antibody (C-Term) (STJ98549)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig/Sheep

£144.00 - £348.00

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Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.

Anti-SMAD5 antibody (C-Term) [4B10-B10-B6] (STJ99081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/FC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts from K562 cells, using Smad5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SMAD5 antibody (Internal) (STJ96452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of lysates from A549 cells using [KD Validated]Smad6 Rabbit polyclonal antibody (STJ11105286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD6 antibody (231-330) (STJ11105286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Immunofluorescence analysis of NIH-3T3 cells using Smad6 antibody (STJ116949) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD6 antibody (257-496) (STJ116949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Smad6 antibody (STJ25610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SMAD6 antibody (257-496) (STJ25610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Smad6 antibody in paraffin-embedded human lung carcinoma tissue.

Anti-SMAD6 antibody (323-372 aa) (STJ95703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis using Smad6 monoclonal antibody against A431 (1) , A431 (2) , HeLa (3) and Jurkat (4) cell lysate.

Anti-SMAD6 antibody [5H3] (STJ98387)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from SW480 cells, using Smad7 Rabbit pAb (STJ29837) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-SMAD7 antibody (220-390) (STJ29837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Smad7 antibody (STJ114223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMAD7 antibody (220-420) (STJ114223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SMAD7 antibody (N-Term) (STJA0005586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£319.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SMAD7 antibody (STJ192434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Goat/Sheep

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human placenta using Smad9 Rabbit polyclonal antibody (STJ29654) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD9 antibody (1-300) (STJ29654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Smad9 antibody (209-226 aa) (STJ11106980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysate from K562 cells, using Brg-1 antibody.

Anti-SMARCA4 antibody (1565-1614 aa) (STJ91889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using BRG1/SMARCA4 antibody (STJ11101695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SMARCA4 antibody (200-300) [S5MR] (STJ11101695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of HeLa cells using BRG1/BRG1/SMARCA4 antibody (STJ113555).

Anti-SMARCA4 antibody (30-130) (STJ113555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g BRG1/SMARCA4 antibody (STJ25612). Western blot was performed from the immunoprecipitate using BRG1/SMARCA4 antibody (STJ25612) at a dilution of 1:1000.

Anti-SMARCA4 antibody (680-770) (STJ25612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot detection of BRG1 in Hela NE, F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.

Anti-SMARCA4 antibody [6D7-F7-B6] (STJ99234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse

£144.00 - £348.00

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Anti-SMC1A antibody (1-130) (STJ116171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using SMC1 antibody (STJ29088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMC1A antibody (1-130) (STJ29088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of HeLa cells using SMC1 Rabbit monoclonal antibody (STJ11102132, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-SMC1A antibody (1134-1233) [S2MR] (STJ11102132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of 293 cells using SMC1 Polyclonal Antibody

Anti-SMC1A antibody (931-980 aa) (STJ95705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot detection of SMC1A (C-term) in MOLT-4, U87 MG and HelaNE cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.

Anti-SMC1A antibody (C-Term) [4C5-C8-A11] (STJ99054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using SMC1 monoclonal antibody against HeLa nuclear extract.

Anti-SMC1A antibody (N-Term) (STJ98551)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Pig

£144.00 - £348.00

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Western blot detection of SMC1A (N-terminus) in HelaNE, Jurkat and A431 cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.

Anti-SMC1A antibody (N-Term) [8E6-A4-F3] (STJ99053)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/FC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using SMC1 monoclonal antibody against K562 (1) , Jurkat (2) and A549 (3) cell lysate.

Anti-SMC1A antibody [5B6] (STJ98388)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-SMC3 antibody (1010-1090 aa) (STJ190157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse spleen using 3 Mu g SMC3 antibody (STJ11100356). Western blot was performed from the immunoprecipitate using SMC3 antibody (STJ11100356) at a dilution of 1:1000.

Anti-SMC3 antibody (909-1199) (STJ11100356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-SMO antibody (28-233 aa) (STJ11106067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of Mouse brain, using SMO antibody (STJ29901) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SMO antibody (28-787) (STJ29901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from HUVEC cells, using SMO Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-SMO antibody (68-117 aa) (STJ95710)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-smooth muscle Myosin heavy chain 11 antibody [SM5002] (STJA0005002)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of various lysates using SMPD2 Rabbit polyclonal antibody (STJ25629) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMPD2 antibody (150-250) (STJ25629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using SMPD3 antibody (STJ112235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMPD3 antibody (416-655) (STJ112235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SMPD3 antibody (C-Term) (STJA0005587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-SMURF1 antibody (150-250 aa) (STJ11106213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-SMURF1 antibody (Internal Region) (STJ13100358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-SMURF1 antibody (STJA0009791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of SW480 cells, using SMURF2 antibody (STJ112605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMURF2 antibody (150-250) (STJ112605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of Mouse lung using 3 Mu g SMURF2 antibody (STJ11101803). Western blot was performed from the immunoprecipitate using SMURF2 antibody (STJ11101803) at a dilution of 1:1000.

Anti-SMURF2 antibody (201-290) [S3MR] (STJ11101803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SMURF2 antibody (505-555 aa) (STJ195579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293F, using Snail antibody (STJ113375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SNAI1 antibody (200-267) (STJ113375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Snail antibody (STJ29909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SNAI1 antibody (200-267) (STJ29909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of rat-heart tissue. 1, SNAI 1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-SNAI1 antibody (215-264 aa) (STJ95716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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