| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.58ng/mL |
| Detection Limit: |
1.56-100ng/mL |
| Short Description: |
This PFKL Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Pfkl |
| Gene ID: |
25741 |
| Uniprot ID: |
PFKAL_RAT |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation. |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. |
| Protein Name | Atp-Dependent 6-Phosphofructokinase - Liver TypeAtp-PfkPfk-L6-Phosphofructokinase Type BPhosphofructo-1-Kinase Isozyme BPfk-BPhosphohexokinase |
| Database Links | Reactome: R-RNO-6798695Reactome: -RNO-70171 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Atp-Dependent 6-Phosphofructokinase - Liver Type ELISA kitAtp-Pfk ELISA kitPfk-L ELISA kit6-Phosphofructokinase Type B ELISA kitPhosphofructo-1-Kinase Isozyme B ELISA kitPfk-B ELISA kitPhosphohexokinase ELISA kitPfkl ELISA kitPfk-l ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
