| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This rat PDGFB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Pdgfb |
| Gene ID: |
24628 |
| Uniprot ID: |
PDGFB_RAT |
| Specificity: |
This kit recognizes Rat PDGFB in samples. No significant cross-reactivity or interference between Rat PDGFB and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in a distinct subpopulation of smooth muscle cells in injured arteries. |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. |
| Protein Name | Platelet-Derived Growth Factor Subunit BPdgf Subunit BPdgf-2Platelet-Derived Growth Factor B ChainPlatelet-Derived Growth Factor Beta Polypeptide |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-1257604Reactome: -RNO-186763Reactome: -RNO-186797Reactome: -RNO-5673001Reactome: -RNO-6811558 |
| Cellular Localisation | SecretedReleased By Platelets Upon Wounding |
| Alternative CLIA Names | Platelet-Derived Growth Factor Subunit B CLIA kitPdgf Subunit B CLIA kitPdgf-2 CLIA kitPlatelet-Derived Growth Factor B Chain CLIA kitPlatelet-Derived Growth Factor Beta Polypeptide CLIA kitPdgfb CLIA kit |
| Specificity | This kit recognizes Rat PDGF-B in samples. No significant cross-reactivity or interference between Rat PDGF-B and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-113 | 107 |
| EDTA plasma(n=8) | 96-110 | 103 |
| Cell culture media(n=8) | 92-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.42 | 82.57 | 383.97 | 38.51 | 87.49 | 388.90 |
| Standard deviation | 3.92 | 9.48 | 31.83 | 4.18 | 9.41 | 24.00 |
| CV (%) | 10.48 | 11.48 | 8.29 | 10.85 | 10.76 | 6.17 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
