| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This rat OPN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Spp1 |
| Gene ID: |
25353 |
| Uniprot ID: |
OSTP_RAT |
| Specificity: |
This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1Secreted Phosphoprotein 1Spp-1 |
| Database Links | Reactome: R-RNO-1474228Reactome: -RNO-186797Reactome: -RNO-216083Reactome: -RNO-381426Reactome: -RNO-8957275 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Osteopontin CLIA kitBone Sialoprotein 1 CLIA kitSecreted Phosphoprotein 1 CLIA kitSpp-1 CLIA kitSpp1 CLIA kit2b7 CLIA kitSpp-1 CLIA kit |
| Specificity | This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 94 |
| EDTA plasma(n=8) | 92-103 | 97 |
| Cell culture media(n=8) | 100-112 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.96 | 2.75 | 6.81 | 0.92 | 2.76 | 6.83 |
| Standard deviation | 0.12 | 0.29 | 0.67 | 0.09 | 0.31 | 0.60 |
| CV (%) | 12.50 | 10.55 | 9.84 | 9.78 | 11.23 | 8.78 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
