| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This Fndc5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Fndc5 |
| Gene ID: |
260327 |
| Uniprot ID: |
FNDC5_RAT |
| Specificity: |
This kit recognizes Rat FNDC5 in samples. No significant cross-reactivity or interference between Rat FNDC5 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Irisin: Mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. |
| Protein Name | Fibronectin Type Iii Domain-Containing Protein 5 Cleaved Into - Irisin |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPeroxisome MembraneImported In Peroxisomes Through The Pex5 Receptor PathwayIrisin: SecretedDetected In The Blood Of Individuals Subjected To Endurance Exercise |
| Alternative ELISA Names | Fibronectin Type Iii Domain-Containing Protein 5 Cleaved Into - Irisin ELISA kitFndc5 ELISA kit |
| Specificity | This kit recognizes Rat FNDC5 in samples. No significant cross-reactivity or interference between Rat FNDC5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-97 | 92 |
| EDTA plasma (n=8) | 88-101 | 94 |
| Cell culture media (n=8) | 88-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 202.57 | 612.64 | 1712.14 | 186.18 | 576.51 | 1563.28 |
| Standard deviation | 10.66 | 25.91 | 89.89 | 9.55 | 27.90 | 73.16 |
| CV (%) | 5.26 | 4.23 | 5.25 | 5.13 | 4.84 | 4.68 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
