• Mouse Fndc5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA (STJE0004491)
  • Mouse Fndc5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA (STJE0004491)

Mouse Fndc5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA (STJE0004491)

SKU:
STJE0004491

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Fndc5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Fndc5
Gene ID: 384061
Uniprot ID: FNDC5_MOUSE
Specificity: This kit recognizes Mouse FNDC5 in samples. No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated.
Function Irisin: Mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.
Protein Name Fibronectin Type Iii Domain-Containing Protein 5
Fibronectin Type Iii Repeat-Containing Protein 2
Peroxisomal Protein
Pep Cleaved Into - Irisin
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Peroxisome Membrane
Secreted
Imported In Peroxisomes Through The Pex5 Receptor Pathway
Irisin: Secreted
Detected In The Blood Of Individuals Subjected To Endurance Exercise
Alternative ELISA Names Fibronectin Type Iii Domain-Containing Protein 5 ELISA kit
Fibronectin Type Iii Repeat-Containing Protein 2 ELISA kit
Peroxisomal Protein ELISA kit
Pep Cleaved Into - Irisin ELISA kit
Fndc5 ELISA kit
Frcp2 ELISA kit
Pep ELISA kit
Specificity This kit recognizes Mouse FNDC5 in samples. No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 96
EDTA plasma (n=8) 93-104 98
Cell culture media (n=8) 88-99 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 194.11 575.25 1915.16 202.17 619.86 2032.21
Standard deviation 12.64 31.70 63.97 12.78 30.81 68.89
CV (%) 6.51 5.51 3.34 6.32 4.97 3.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance