• Human FNDC5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA (STJE0004132)

Human FNDC5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA (STJE0004132)

SKU:
STJE0004132

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This FNDC5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: FNDC5
Gene ID: 252995
Uniprot ID: FNDC5_HUMAN
Specificity: This kit recognizes Human Irisin in samples. No significant cross-reactivity or interference between Human Irisin and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated.
Function Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Protein Name Fibronectin Type Iii Domain-Containing Protein 5
Fibronectin Type Iii Repeat-Containing Protein 2 Cleaved Into - Irisin
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Peroxisome Membrane
Imported In Peroxisomes Through The Pex5 Receptor Pathway
Irisin: Secreted
Detected In The Blood Of Individuals Subjected To Endurance Exercise
Alternative ELISA Names Fibronectin Type Iii Domain-Containing Protein 5 ELISA kit
Fibronectin Type Iii Repeat-Containing Protein 2 Cleaved Into - Irisin ELISA kit
FNDC5 ELISA kit
FRCP2 ELISA kit
Specificity This kit recognizes Human Irisin in samples. No significant cross-reactivity or interference between Human Irisin and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma (n=8) 94-109 102
Cell culture media (n=8) 93-102 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 40.28 104.39 474.47 42.15 100.83 403.90
Standard deviation 2.15 5.18 23.82 2.15 4.59 18.42
CV (%) 5.34 4.96 5.02 5.10 4.55 4.56
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance