| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
25.13pg/mL |
| Detection Limit: |
93.7-6000pg/mL |
| Short Description: |
This ENK Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Penk |
| Gene ID: |
29237 |
| Uniprot ID: |
PENK_RAT |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | Expressed in brain, heart and testis. |
| Post Translational Modifications | Proenkephalin-A is cleaved by CTSL to generate Met-enkephalin. Met-enkephalin: Processed and degraded by ACE. Leu-enkephalin: Processed and degraded by ACE. Met-enkephalin-Arg-Gly-Leu: Probably cleaved by ACE. Met-enkephalin-Arg-Phe: Processed by ACE to generate Met-enkephalin in the nucleus accumbens of the brain. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. |
| Function | Met-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Leu-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Met-enkephalin-Arg-Phe: Met-enkephalin-Arg-Phe neuropeptide acts as a strong ligand of Mu-type opioid receptor OPRM1. Met-enkephalin-Arg-Phe-binding to OPRM1 in the nucleus accumbens of the brain increases activation of OPRM1, leading to long-term synaptic depression of glutamate release. PENK(114-133): Increases glutamate release in the striatum and decreases GABA concentration in the striatum. PENK(239-260): Increases glutamate release in the striatum. |
| Protein Name | Proenkephalin-A Cleaved Into - Synenkephalin - Met-EnkephalinOpioid Growth FactorOgf - Penk(114-133 - Penk(143-185 - Met-Enkephalin-Arg-Gly-Leu - Leu-Enkephalin - Penk(239-260 - Met-Enkephalin-Arg-Phe |
| Database Links | Reactome: R-RNO-375276Reactome: -RNO-381426Reactome: -RNO-418594Reactome: -RNO-8957275 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory VesicleChromaffin Granule Lumen |
| Alternative ELISA Names | Proenkephalin-A Cleaved Into - Synenkephalin - Met-Enkephalin ELISA kitOpioid Growth Factor ELISA kitOgf - Penk(114-133 - Penk(143-185 - Met-Enkephalin-Arg-Gly-Leu - Leu-Enkephalin - Penk(239-260 - Met-Enkephalin-Arg-Phe ELISA kitPenk ELISA kitPenk-rs ELISA kitPenk1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
