| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This rat ATXN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Atxn1 |
| Gene ID: |
25049 |
| Uniprot ID: |
ATX1_RAT |
| Specificity: |
This kit recognizes Rat ATXN1 in samples. No significant cross-reactivity or interference between Rat ATXN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitinated by UBE3A, leading to its degradation by the proteasome. Phosphorylation at Ser-749 increases the pathogenicity of proteins with an expanded polyglutamine tract. Sumoylation is dependent on nuclear localization and phosphorylation at Ser-749. It is reduced in the presence of an expanded polyglutamine tract. |
| Function | Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved brain development. |
| Protein Name | Ataxin-1Spinocerebellar Ataxia Type 1 Protein Homolog |
| Cellular Localisation | CytoplasmNucleusColocalizes With Usp7 In The Nucleus |
| Alternative CLIA Names | Ataxin-1 CLIA kitSpinocerebellar Ataxia Type 1 Protein Homolog CLIA kitAtxn1 CLIA kitSca1 CLIA kit |
| Specificity | This kit recognizes Rat ATXN1 in samples. No significant cross-reactivity or interference between Rat ATXN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-97 | 90 |
| EDTA plasma(n=8) | 93-106 | 100 |
| Cell culture media(n=8) | 100-114 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 214.98 | 479.72 | 1712.22 | 216.50 | 466.51 | 1840.05 |
| Standard deviation | 21.18 | 57.18 | 129.27 | 25.74 | 40.40 | 170.94 |
| CV (%) | 9.85 | 11.92 | 7.55 | 11.89 | 8.66 | 9.29 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
