| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This Amh Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Amh |
| Gene ID: |
25378 |
| Uniprot ID: |
MIS_RAT |
| Specificity: |
This kit recognizes Rat AMH in samples. No significant cross-reactivity or interference between Rat AMH and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Preproprotein is proteolytically processed to generate N- and C-terminal cleavage products that homodimerize and associate to form a biologically active non-covalent complex. Binding of the non-covalent complex to AMHR2 induces dissociation of the pro-region from the mature C-terminal dimer. The N-terminal portion of the protein, despite having no intrinsic activity, has the role of amplifying the activity of the C-terminus. |
| Function | Plays an important role in several reproductive functions. Induces Muellerian duct regression during male fetal sexual differentiation and plays a role in Leydig cell differentiation and function. In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles. AMH signals by binding to a specific type-II receptor, AMHR2, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A), and recruiting SMAD proteins that are translocated to the nucleus to regulate target gene expression. |
| Protein Name | Muellerian-Inhibiting FactorAnti-Muellerian HormoneAmhMuellerian-Inhibiting SubstanceMis |
| Database Links | Reactome: R-RNO-201451 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Muellerian-Inhibiting Factor ELISA kitAnti-Muellerian Hormone ELISA kitAmh ELISA kitMuellerian-Inhibiting Substance ELISA kitMis ELISA kitAmh ELISA kit |
| Specificity | This kit recognizes Rat AMH in samples. No significant cross-reactivity or interference between Rat AMH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-99 | 95 |
| EDTA plasma (n=8) | 90-99 | 95 |
| Cell culture media (n=8) | 92-107 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 164.78 | 488.04 | 1854.15 | 191.52 | 482.82 | 1632.48 |
| Standard deviation | 9.72 | 23.91 | 79.73 | 11.17 | 21.82 | 78.20 |
| CV (%) | 5.90 | 4.90 | 4.30 | 5.83 | 4.52 | 4.79 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
