Phospho Antibodies | St John's Laboratory

Anti-Phospho-PDHA1-Ser293 antibody [R46-5D-6] (STJA0035679)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western Blot analysis of 1 HepG2 cell 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PDHA1-Ser293/291 antibody (STJA0009914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0005522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$507.15

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0041348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

$748.65

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PDK1-Thr338 antibody (STJA0009880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDK1-Tyr-9 antibody (STJA0005521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$507.15

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Anti-Phospho-PDK1-Tyr-9 antibody (STJA0041333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PDK1-Tyr243 antibody (STJA0009879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of K562 cells using Phospho-PDLIM5 (Y251) Polyclonal Antibody

Anti-Phospho-PDLIM5-Tyr251 antibody (STJ90383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (STJ22329) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-PDPK1-S241 antibody (STJ22329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (STJ29323) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PDPK1-S241 antibody (STJ29323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (STJ11105841) at1:2000 dilution. PC-3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-Phospho-PDPK1-S241 antibody [S5841RM] (STJ11105841)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Ser241 antibody (210-259 aa) (STJ90382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDPK1-Ser241 antibody [PT0869R] (STJA0039151)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using PDK1 (Phospho-Tyr9) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Tyr9 antibody (1-50 aa) (STJ91192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of Hela cell, 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PDX1-Ser61 antibody (STJA0010002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PDX1-Ser66 antibody (STJA0009971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using Phospho-PEA15-S104 Rabbit pAb (STJ11101012) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-PEA15-S104 antibody (STJ11101012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa and C6 cells, using Phospho-PEA15-S104 Rabbit pAb (STJ117886) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PEA15-S104 antibody (STJ117886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded human heart, using PEA-15 (Phospho-Ser104) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PEA15-Ser104 antibody (70-119 aa) (STJ90861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of COLO 3T3 using p-PEA-15 (S116) antibody. Antibody was diluted at 1:500

Anti-Phospho-PEA15-Ser116 antibody (81-130 aa) (STJ90829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-PECAM1-Tyr690 antibody (STJA0010047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HepG2 cells, using PECAM-1 (Phospho-Tyr713) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PECAM1-Tyr713 antibody (686-735 aa) (STJ90690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of 3T3 cells using Phospho-Per2 (S662) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PER2-Ser662 antibody (636-685 aa) (STJ90924)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-PERK-Thr980 antibody [PT0868R] (STJA0039776)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Mouse/Rat

$393.30 - $745.20

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Western blot analysis of lysates from HeLa cells, using Phospho-PFKFB2-S483 Rabbit pAb (STJ117882) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-PFKFB2-S483 antibody (STJ117882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western Blot analysis of K562 mouse brain using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-PFKFB2-Ser466 antibody (STJA0010083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PFKFB2-Ser483 antibody (451-500 aa) (STJ90871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PFN1-Tyr-129 antibody (STJA0005550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$507.15

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Anti-Phospho-PFN1-Tyr-138 antibody (STJA0005548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$507.15

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Western blot analysis of Maize 1) Dark, 2) Light, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Phospho-PGC-Ser15 antibody (STJ197449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Plant

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-PR (S190) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PGR-Ser190 antibody (161-210 aa) (STJ90395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

$67.62 - $360.18

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Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).

Anti-Phospho-PGR-Ser190 antibody [1154] (STJA0003755)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

$674.13

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Western blot analysis of 293 cells using Phospho-PR (S294) Polyclonal Antibody

Anti-Phospho-PGR-Ser294 antibody (261-310 aa) (STJ90691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PGR-Ser294 antibody [608] (STJA0003756)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Non-Human Primates

$674.13

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PGR-Ser345 antibody (STJ196391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of 293 cells using Phospho-PR (S400) Polyclonal Antibody

Anti-Phospho-PGR-Ser400 antibody (371-420 aa) (STJ90692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-PHGDH-Thr204 antibody (STJA0009878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PHGDH-Thr207 antibody (STJA0009877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Phospho-Tyrosine antibody [6G6-3D7-7A7] (STJA0032875)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

$184.23 - $388.47

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Anti-Phospho-Phospho-Tyrosine antibody [R06-9L4] (STJA0036315)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-Phospholamban-Ser16 antibody [R03-1I4] (STJA0034720)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-Phospho-PI3 Kinase p55-Y199-Y199 antibody [SRM15133] (STJ11109863)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

$0.00

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Anti-Phospho-PI3 Kinase p85/p55-Tyr458+Tyr199 antibody [PT0750R] (STJA0040075)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunofluorescence analysis of human-lung tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PI3K p85/p55-Tyr467/199 antibody (436-485 aa) (STJ90384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Pig

$67.62 - $360.18

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Anti-Phospho-PI3KP85 Alpha-Y467+P85 Beta-Y464+P55 Gamma-Y199 antibody {ABflo® 488} (STJ11109141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$0.00

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Anti-Phospho-PI3KP85 Alpha-Y467+P85 Beta-Y464+P55 Gamma-Y199 antibody {ABflo® 647} (STJ11109142)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$0.00

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Western blot analysis of lysates from wild type (WT) and 293T cells were transfected with SRC (Y530F) , using Phospho-PI3KP85 Alpha/ Gamma/ Beta-Y467/Y199/Y464 Rabbit pAb (STJ11107668) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PI3KP85 Alpha/P55 Gamma/P85 Beta-Y467/Y199/Y464 antibody (STJ11107668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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