Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using PHF19 Rabbit pAb (STJ110368) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PHF19 antibody (1-207) (STJ110368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using PHF3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PHF3 antibody (1990-2039 aa) (STJ95063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PHGDH antibody (STJ112490). Western blot was performed from the immunoprecipitate using PHGDH antibody (STJ112490) at a dilution of 1:1000.

Anti-PHGDH antibody (264-533) (STJ112490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of MCF-7 cells using PHIP antibody (STJ29287).

Anti-PHIP antibody (1542-1821) (STJ29287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts from normal (control) and PHKG2 knockout (KO) 293T cells, using PHKG2 antibody (STJ11100810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PHKG2 antibody (237-406) (STJ11100810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of U-2 OS cells using PHKG2 Rabbit polyclonal antibody (STJ29476) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PHKG2 antibody (237-406) (STJ29476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from PC-3 cells using PHLDA2 rabbit polyclonal antibody (STJ28000) at 1:4000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-PHLDA2 antibody (1-152) (STJ28000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human placenta, using PHLA2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PHLDA2 antibody (81-130 aa) (STJ96131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-PHLDA2/IPL antibody (C-Term) (STJ70472)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC/FC

Reactivity :

Human

$421.88

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Western blot analysis of various cells using PHLDA3 Polyclonal Antibody

Anti-PHLDA3 antibody (21-70 aa) (STJ95068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using PHLPP1 Rabbit pAb (STJ111739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PHLPP1 antibody (1160-1428) (STJ111739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of L929 cells using PHLPP2 Rabbit polyclonal antibody (STJ11100175) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PHLPP2 antibody (1250-1323) (STJ11100175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence –anti-pHluorin antibody using hCEC cells transduced with GFP-CAV1; cells were fixed with methanol and anti-pHluorin at 1/250

Anti-pHluorin antibody (STJ140262)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

$182.93 - $532.58

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STJ71424 (1µg/ml) staining of Human Spleen (A) , Tonsil (B) and (0. 1ug/ml) PBM (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Phorbolin 1/APOBEC3A antibody (Internal) (STJ71424)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human

$421.88

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-4E-BP1-Thr69 antibody (35-84 aa) (STJ90163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Thr735 antibody (701-750 aa) (STJ90579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ABL1-Tyr204 antibody (156-205 aa) (STJ90452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Tyr245 antibody (196-245 aa) (STJ90694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Abl (Phospho-Tyr412) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Abl1/2-Tyr393/439 antibody (361-410 aa) (STJ90779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Immunofluorescence analysis of A549 cells, using Abl (Phospho-Tyr393/412) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Abl1/2-Tyr393/439 antibody (406-455 aa) (STJ90164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-ACC Alpha (S80) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ACACA-Ser80 antibody (46-95 aa) (STJ90755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 3T3 using p-ACC Alpha (S80) antibody. Antibody was diluted at 1:1000

Anti-Phospho-ACACA-Ser80 antibody (46-95 aa) (STJ90780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-ACAP1-Ser554 antibody (520-569 aa) (STJ91177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-ACC1/ACC2-S79 antibody [SRM] (STJ11107725)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-ACK1 antibody (250-300) (STJ502083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Anti-Phospho-ACK1 antibody (250-300) {Biotin} (STJ502084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$789.08

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Anti-Phospho-ACK1 antibody (300-400) {FITC} (STJ502085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$789.08

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Western blot analysis of lysates from COS7 cells treated with Calyculin 50nM 30', using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ACLY-Ser455 antibody (420-469 aa) (STJ90846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of IREB1 (Phospho-Ser138) Antibody. The lane on the right is blocked with the IREB1 (Phospho-Ser138) peptide.

Anti-Phospho-ACO1-Ser138 antibody (106-155 aa) (STJ91163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293 cells using Phospho-IRP-1 (S711) Polyclonal Antibody

Anti-Phospho-ACO1-Ser711 antibody (681-730 aa) (STJ90914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Actin-Alpha/Gamma-Tyr55/53 antibody (21-70 aa) (STJ91137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from K562 cells treated with UV 5', using ADAM 17 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ADAM17-Thr735 antibody (701-750 aa) (STJ90495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Thr445) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADD1-Thr445 antibody (411-460 aa) (STJ91115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Adducin Alpha/Beta (S726/713) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-Adducin Alpha/Beta-Ser726/713 antibody (688-737 aa) (STJ91014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADRB2-Ser346 antibody (321-370 aa) (STJ91102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADRB2-Ser355/S356 antibody (331-380 aa) (STJ90872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of HepG2 cells using Phospho-Ah Receptor (S36) Polyclonal Antibody

Anti-Phospho-Ah Receptor-Ser36 antibody (2-51 aa) (STJ90873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of rat-liver tissue. 1, Akt (phospho Ser473) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Ser473 antibody (431-480 aa) (STJ90166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

$64.80 - $340.20

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Immunofluorescence analysis of human-lung tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Thr308 antibody (STJ90750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using AKT1/2/3 (Phospho-Tyr315/316/312) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Akt-Tyr315 antibody (281-330 aa) (STJ91195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded Human brain using Phospho-Akt-Y315 Rabbit pAb (STJ11105776) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-Akt-Y315 antibody (STJ11105776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [6F8] (STJ97726)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$64.80 - $340.20

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Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$64.80 - $340.20

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser246 antibody (212-261 aa) (STJ90165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

Choose Options