| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
PAR1 Blocking Peptide for STJ502112 is synthetically produced from the 390-440 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
F2r |
| Gene ID: |
14062 |
| Uniprot ID: |
PAR1_MOUSE |
| Immunogen Region: |
390-440 |
| Specificity: |
This blocking peptide is recommended for use in combination with PAR1 antibody, STJ502112 |
| Immunogen: |
Synthetic peptide taken within amino acid region 390-440 on mouse PAR1 protein. |
| Post Translational Modifications | Proteolytic cleavage by thrombin generates a new N-terminus that functions as a tethered ligand. Also proteolytically cleaved by cathepsin CTSG. Phosphorylated in the C-terminal tail.probably mediating desensitization prior to the uncoupling and internalization of the receptor. |
| Function | High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores. The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores. In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. May play a role in platelets activation and in vascular development. Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells. |
| Peptide Name | Proteinase-Activated Receptor 1Par-1Thrombin Receptor |
| Database Links | Reactome: R-MMU-140875Reactome: -MMU-375276Reactome: -MMU-416476Reactome: -MMU-456926 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Peptide Names | Proteinase-Activated Receptor 1 proteinPar-1 proteinThrombin Receptor proteinF2r proteinCf2r proteinPar1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
