Applications: | ELISA |
Reactivity: | Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Sensitivity: | 0.107ng/mL |
Detection Limit: | 0.312-20ng/mL |
Short Description: | This XRCC6 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
Assay Time: | 3 hrs |
Gene Symbol: | Xrcc6 |
Gene ID: | 14375 |
Uniprot ID: | XRCC6_MOUSE |
Immunogen Region: | Ready-To-Use |
Sample Type: | tissue homogenates or other biological fluids. |
Tissue Specificity | |
Post Translational Modifications | Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-49 does not affect DNA repair. ADP-ribosylated by PARP3. |
Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
Protein Name | X-Ray Repair Cross-Complementing Protein 6 5'-Deoxyribose-5-Phosphate Lyase Ku70 5'-Drp/Ap Lyase Ku70 Atp-Dependent Dna Helicase 2 Subunit 1 Atp-Dependent Dna Helicase Ii 70 Kda Subunit Ctc Box-Binding Factor 75 Kda Subunit Ctc75 Ctcbf Dna Repair Protein Xrcc6 Ku Autoantigen Protein P70 Homolog Ku70 |
Database Links | Reactome: R-MMU-5693571 Reactome: -MMU-6798695 |
Cellular Localisation | Nucleus Chromosome |
Alternative ELISA Names | X-Ray Repair Cross-Complementing Protein 6 ELISA kit 5'-Deoxyribose-5-Phosphate Lyase Ku70 ELISA kit 5'-Drp/Ap Lyase Ku70 ELISA kit Atp-Dependent Dna Helicase 2 Subunit 1 ELISA kit Atp-Dependent Dna Helicase Ii 70 Kda Subunit ELISA kit Ctc Box-Binding Factor 75 Kda Subunit ELISA kit Ctc75 ELISA kit Ctcbf ELISA kit Dna Repair Protein Xrcc6 ELISA kit Ku Autoantigen Protein P70 Homolog ELISA kit Ku70 ELISA kit Xrcc6 ELISA kit G22p1 ELISA kit Ku70 ELISA kit |
output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance