• Mouse Sftpa1 (Pulmonary surfactant-associated protein A) Sandwich ELISA (STJE0004932)

Mouse Sftpa1 (Pulmonary surfactant-associated protein A) Sandwich ELISA (STJE0004932)

SKU:
STJE0004932

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This Sftpa1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Sftpa1Uniprot ID=SFTPA_MOUSE"
Specificity: This kit recognizes Mouse SP-A1 in samples. No significant cross-reactivity or interference between Mouse SP-A1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Pulmonary Surfactant-Associated Protein A
Psap
Psp-A
Sp-A
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Surface Film
Alternative ELISA Names Pulmonary Surfactant-Associated Protein A ELISA kit
Psap ELISA kit
Psp-A ELISA kit
Sp-A ELISA kit
Sftpa1 ELISA kit
Sftp-1 ELISA kit
Sftp1 ELISA kit
Sftpa ELISA kit
Specificity This kit recognizes Mouse SP-A1 in samples. No significant cross-reactivity or interference between Mouse SP-A1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 98
EDTA plasma (n=8) 85-99 91
Cell culture media (n=8) 87-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.05 2.63 7.39 0.96 2.74 7.22
Standard deviation 0.06 0.11 0.27 0.06 0.14 0.27
CV (%) 5.71 4.18 3.65 6.25 5.11 3.74
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance