| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This SFTPA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SFTPA1 |
| Gene ID: |
653509 |
| Uniprot ID: |
SFTA1_HUMAN |
| Specificity: |
This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages. (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule. Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages. (Microbial infection) Binds M.pneumoniae CARDS toxin, serves as one receptor for this pathogen. When SFTPA1 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen. |
| Protein Name | Pulmonary Surfactant-Associated Protein A1Psp-APspaSp-ASp-A135 Kda Pulmonary Surfactant-Associated ProteinAlveolar Proteinosis ProteinCollectin-4 |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-168179Reactome: R-HSA-391160Reactome: R-HSA-5683826Reactome: R-HSA-5686938Reactome: R-HSA-5688849Reactome: R-HSA-5688890 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixSurface Film |
| Alternative ELISA Names | Pulmonary Surfactant-Associated Protein A1 ELISA kitPsp-A ELISA kitPspa ELISA kitSp-A ELISA kitSp-A1 ELISA kit35 Kda Pulmonary Surfactant-Associated Protein ELISA kitAlveolar Proteinosis Protein ELISA kitCollectin-4 ELISA kitSFTPA1 ELISA kitCOLEC4 ELISA kitPSAP ELISA kitSFTP1 ELISA kitSFTPA ELISA kitSFTPA1B ELISA kit |
| Specificity | This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-98 | 92 |
| EDTA plasma (n=8) | 85-98 | 91 |
| Cell culture media (n=8) | 88-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.86 | 136.08 | 414.87 | 48.73 | 132.09 | 424.02 |
| Standard deviation | 2.60 | 7.61 | 16.18 | 2.93 | 5.76 | 20.01 |
| CV (%) | 5.01 | 5.59 | 3.90 | 6.01 | 4.36 | 4.72 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
