| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
23.44pg/mL |
| Detection Limit: |
39.06~2500pg/mL |
| Short Description: |
This Hnf4a Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Hnf4a |
| Gene ID: |
15378 |
| Uniprot ID: |
HNF4A_MOUSE |
| Specificity: |
This kit recognizes Mouse HNF4a in samples. No significant cross-reactivity or interference between Mouse HNF4a and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated on tyrosine residue(s).phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. |
| Function | Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitatating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells. |
| Protein Name | Hepatocyte Nuclear Factor 4-AlphaHnf-4-AlphaNuclear Receptor Subfamily 2 Group A Member 1Transcription Factor 14Tcf-14Transcription Factor Hnf-4 |
| Database Links | Reactome: R-MMU-383280 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Hepatocyte Nuclear Factor 4-Alpha ELISA kitHnf-4-Alpha ELISA kitNuclear Receptor Subfamily 2 Group A Member 1 ELISA kitTranscription Factor 14 ELISA kitTcf-14 ELISA kitTranscription Factor Hnf-4 ELISA kitHnf4a ELISA kitHnf-4 ELISA kitHnf4 ELISA kitNr2a1 ELISA kitTcf14 ELISA kit |
| Specificity | This kit recognizes Mouse HNF4a in samples. No significant cross-reactivity or interference between Mouse HNF4a and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma (n=8) | 89-103 | 95 |
| Cell culture media (n=8) | 91-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 126.81 | 348.80 | 1053.83 | 137.37 | 338.03 | 978.80 |
| Standard deviation | 7.19 | 17.89 | 33.72 | 7.32 | 17.81 | 29.56 |
| CV (%) | 5.67 | 5.13 | 3.20 | 5.33 | 5.27 | 3.02 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
