Mouse HGF (Hepatocyte Growth Factor) Sandwich ELISA (STJE0019729)

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STJE0019729

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56-100pg/mL
Short Description: This HGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Hgf
Gene ID: 15234
Uniprot ID: HGF_MOUSE
Specificity: This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.
Protein Name Hepatocyte Growth Factor
Hepatopoietin-A
Scatter Factor
Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain
Database Links Reactome: R-MMU-114608
Reactome: -MMU-1257604
Reactome: -MMU-5673001
Reactome: -MMU-6806942
Reactome: -MMU-6807004
Reactome: -MMU-6811558
Reactome: -MMU-8851805
Reactome: -MMU-8851907
Reactome: -MMU-8865999
Reactome: -MMU-8874081
Reactome: -MMU-8875513
Reactome: -MMU-8875555
Reactome: -MMU-8875656
Reactome: -MMU-8875791
Reactome: -MMU-9734091
Alternative ELISA Names Hepatocyte Growth Factor ELISA kit
Hepatopoietin-A ELISA kit
Scatter Factor ELISA kit
Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain ELISA kit
Hgf ELISA kit
Specificity This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-109 100
EDTA plasma (n=8) 93-106 100
Cell culture media (n=8) 94-107 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 4.70 9.77 35.91 4.98 9.39 38.13
Standard deviation 0.31 0.52 1.77 0.32 0.54 1.76
CV (%) 6.55 5.28 4.92 6.49 5.74 4.62
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance