| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This mouse FV kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F5 |
| Gene ID: |
14067 |
| Uniprot ID: |
FA5_MOUSE |
| Specificity: |
This kit recognizes Mouse FV in samples. No significant cross-reactivity or interference between Mouse FV and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus). Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity. Activated protein C inactivates factor V and factor Va by proteolytic degradation. |
| Function | Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. |
| Protein Name | Coagulation Factor VActivated Protein C Cofactor Cleaved Into - Coagulation Factor V Heavy Chain - Coagulation Factor V Light Chain |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-140875Reactome: -MMU-204005Reactome: -MMU-381426Reactome: -MMU-5694530Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Coagulation Factor V CLIA kitActivated Protein C Cofactor Cleaved Into - Coagulation Factor V Heavy Chain - Coagulation Factor V Light Chain CLIA kitF5 CLIA kit |
| Specificity | This kit recognizes Mouse FV in samples. No significant cross-reactivity or interference between Mouse FV and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-113 | 105 |
| EDTA plasma(n=8) | 89-105 | 96 |
| Cell culture media(n=8) | 85-97 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.90 | 4.73 | 17.70 | 1.99 | 4.41 | 17.67 |
| Standard deviation | 0.17 | 0.48 | 1.83 | 0.17 | 0.52 | 1.43 |
| CV (%) | 8.95 | 10.15 | 10.34 | 8.54 | 11.79 | 8.09 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
