| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This ACP3/PAP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Acp3 |
| Gene ID: |
56318 |
| Uniprot ID: |
PPAP_MOUSE |
| Specificity: |
This kit recognizes Mouse ACP3/PAP in samples. No significant crossreactivity or interference between Mouse ACP3/PAP and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1: Expressed in salivary gland, thymus and thyroid gland. Isoform 2: Widely expressed in prostate lobes, brain, kidney, liver, lung, muscle, placenta, salivary gland, spleen, thyroid and thymus. Locates to Schwann cells and fibroblasts. Expressed in peptidergic and non-peptidergic nociceptive (pain-sensing) neurons. Preferentially expressed in non-peptidergic doral root ganglia neurons. |
| Function | A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: In addition to its tyrosine phosphatase activity, also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP. This extracellular adenosine leads to a decrease in chronic pain by activating A1R in nociceptive neurons. |
| Protein Name | Prostatic Acid Phosphatase5'-Nucleotidase5'-NtAcid Phosphatase 3Ecto-5'-NucleotidaseFluoride-Resistant Acid PhosphataseFrapProtein Tyrosine Phosphatase Acp3Thiamine MonophosphataseTmpase |
| Database Links | Reactome: R-MMU-6798695 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Type I Membrane ProteinLysosome MembraneAppears To Shuttle Between The Cell Membrane And Intracellular VesiclesColocalizes With Flot1 At Cell Membrane And In Intracellular VesiclesColocalizes With Lamp2 On The Lysosome Membrane |
| Alternative ELISA Names | Prostatic Acid Phosphatase ELISA kit5'-Nucleotidase ELISA kit5'-Nt ELISA kitAcid Phosphatase 3 ELISA kitEcto-5'-Nucleotidase ELISA kitFluoride-Resistant Acid Phosphatase ELISA kitFrap ELISA kitProtein Tyrosine Phosphatase Acp3 ELISA kitThiamine Monophosphatase ELISA kitTmpase ELISA kitAcp3 ELISA kitAcpp ELISA kit |
| Specificity | This kit recognizes Mouse ACP3/PAP in samples. No significant cross-reactivity or interference between Mouse ACP3/PAP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 94 |
| EDTA plasma (n=8) | 86-100 | 93 |
| Cell culture media (n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 5.42 | 15.37 | 45.58 | 5.25 | 15.55 | 47.54 |
| Standard deviation | 0.33 | 0.66 | 2.07 | 0.29 | 0.87 | 2.05 |
| CV (%) | 6.09 | 4.29 | 4.54 | 5.52 | 5.59 | 4.31 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
