| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.057ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This XRCC5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
XRCC5 |
| Gene ID: |
7520 |
| Uniprot ID: |
XRCC5_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | ADP-ribosylated by PARP3. Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. Sumoylated. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks. |
| Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
| Protein Name | X-Ray Repair Cross-Complementing Protein 586 Kda Subunit Of Ku AntigenAtp-Dependent Dna Helicase 2 Subunit 2Atp-Dependent Dna Helicase Ii 80 Kda SubunitCtc Box-Binding Factor 85 Kda SubunitCtc85CtcbfDna Repair Protein Xrcc5Ku80Ku86Lupus Ku Autoantigen Protein P86Nuclear Factor IvThyroid-Lupus AutoantigenTlaaX-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5Double-Strand-Break Rejoining |
| Database Links | Reactome: R-HSA-164843Reactome: R-HSA-1834949Reactome: R-HSA-3270619Reactome: R-HSA-5693571Reactome: R-HSA-6798695 |
| Cellular Localisation | NucleusNucleolusChromosome |
| Alternative ELISA Names | X-Ray Repair Cross-Complementing Protein 5 ELISA kit86 Kda Subunit Of Ku Antigen ELISA kitAtp-Dependent Dna Helicase 2 Subunit 2 ELISA kitAtp-Dependent Dna Helicase Ii 80 Kda Subunit ELISA kitCtc Box-Binding Factor 85 Kda Subunit ELISA kitCtc85 ELISA kitCtcbf ELISA kitDna Repair Protein Xrcc5 ELISA kitKu80 ELISA kitKu86 ELISA kitLupus Ku Autoantigen Protein P86 ELISA kitNuclear Factor Iv ELISA kitThyroid-Lupus Autoantigen ELISA kitTlaa ELISA kitX-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 ELISA kitDouble-Strand-Break Rejoining ELISA kitXRCC5 ELISA kitG22P2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
