• Mouse XRCC5 (X-Ray Repair Cross Complementing 5) Sandwich ELISA Kit (STJE0013130)

Mouse XRCC5 (X-Ray Repair Cross Complementing 5) Sandwich ELISA Kit (STJE0013130)

SKU:
STJE0013130

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.072ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This XRCC5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Xrcc5
Gene ID: 22596
Uniprot ID: XRCC5_MOUSE
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications ADP-ribosylated by PARP3. Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. Sumoylated. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.
Function Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Protein Name X-Ray Repair Cross-Complementing Protein 5
Atp-Dependent Dna Helicase 2 Subunit 2
Atp-Dependent Dna Helicase Ii 80 Kda Subunit
Ctc Box-Binding Factor 85 Kda Subunit
Ctc85
Ctcbf
Dna Repair Protein Xrcc5
Ku Autoantigen Protein P86 Homolog
Ku80
Nuclear Factor Iv
Database Links Reactome: R-MMU-5693571
Reactome: -MMU-6798695
Cellular Localisation Nucleus
Nucleolus
Chromosome
Alternative ELISA Names X-Ray Repair Cross-Complementing Protein 5 ELISA kit
Atp-Dependent Dna Helicase 2 Subunit 2 ELISA kit
Atp-Dependent Dna Helicase Ii 80 Kda Subunit ELISA kit
Ctc Box-Binding Factor 85 Kda Subunit ELISA kit
Ctc85 ELISA kit
Ctcbf ELISA kit
Dna Repair Protein Xrcc5 ELISA kit
Ku Autoantigen Protein P86 Homolog ELISA kit
Ku80 ELISA kit
Nuclear Factor Iv ELISA kit
Xrcc5 ELISA kit
G22p2 ELISA kit
output

Information sourced from Uniprot.org

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