• Human USP8 (Ubiquitin Specific Peptidase 8) Sandwich ELISA Kit (STJE0012956)

Human USP8 (Ubiquitin Specific Peptidase 8) Sandwich ELISA Kit (STJE0012956)

SKU:
STJE0012956

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.101ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This USP8 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: USP8
Gene ID: 9101
Uniprot ID: UBP8_HUMAN
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner. Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.
Function Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controls tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 8
Deubiquitinating Enzyme 8
Ubiquitin Isopeptidase Y
Hubpy
Ubiquitin Thioesterase 8
Ubiquitin-Specific-Processing Protease 8
Database Links Reactome: R-HSA-1358803
Reactome: R-HSA-4641263
Reactome: R-HSA-5689880
Reactome: R-HSA-6807004
Cellular Localisation Cytoplasm
Nucleus
Endosome Membrane
Peripheral Membrane Protein
Cell Membrane
Alternative ELISA Names Ubiquitin Carboxyl-Terminal Hydrolase 8 ELISA kit
Deubiquitinating Enzyme 8 ELISA kit
Ubiquitin Isopeptidase Y ELISA kit
Hubpy ELISA kit
Ubiquitin Thioesterase 8 ELISA kit
Ubiquitin-Specific-Processing Protease 8 ELISA kit
USP8 ELISA kit
KIAA0055 ELISA kit
UBPY ELISA kit
output

Information sourced from Uniprot.org

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