| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human tPA/Tissue-type plasminogen activator/PLAT-C-6×His protein was developed from hek293 cells and has a target region of C-6×His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.2 Mu m filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl2, pH5.5. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLAT |
| Gene ID: |
5327 |
| Uniprot ID: |
TPA_HUMAN |
| Immunogen Region: |
Ser36-Pro562 |
| Immunogen: |
Recombinant Human tPA/Tissue-type plasminogen activator/PLAT Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ser36-Pro562) of human tPA/Tissue-type plasminogen activator/PLAT (Accession #P00750) fu |
| Post Translational Modifications | The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin. N-glycosylation of Asn-152.the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. Characterization of O-linked glycan was studied in Bowes melanoma cell line. |
| Function | Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy. |
| Protein Name | Tissue-Type Plasminogen ActivatorT-PaT-Plasminogen ActivatorTpaAlteplaseReteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-186797Reactome: R-HSA-75205 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Protein Names | Tissue-Type Plasminogen Activator proteinT-Pa proteinT-Plasminogen Activator proteinTpa proteinAlteplase proteinReteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B proteinPLAT protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
