| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
75pg/mL |
| Detection Limit: |
125~8000pg/mL |
| Short Description: |
This human TGFBI kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
TGFBI |
| Gene ID: |
7045 |
| Uniprot ID: |
BGH3_HUMAN |
| Specificity: |
This kit recognizes Human TGF Beta I in samples. No significant cross-reactivity or interference between Human TGF Beta I and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in the corneal epithelium. Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain. |
| Function | Plays a role in cell adhesion. May play a role in cell-collagen interactions. |
| Protein Name | Transforming Growth Factor-Beta-Induced Protein Ig-H3Beta Ig-H3Kerato-EpithelinRgd-Containing Collagen-Associated ProteinRgd-Cap |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixMay Be Associated Both With Microfibrils And With The Cell Surface |
| Alternative CLIA Names | Transforming Growth Factor-Beta-Induced Protein Ig-H3 CLIA kitBeta Ig-H3 CLIA kitKerato-Epithelin CLIA kitRgd-Containing Collagen-Associated Protein CLIA kitRgd-Cap CLIA kitTGFBI CLIA kitBIGH3 CLIA kit |
| Specificity | This kit recognizes Human TGFβI in samples. No significant cross-reactivity or interference between Human TGFβI and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-100 | 92 |
| EDTA plasma(n=8) | 98-112 | 106 |
| Cell culture media(n=8) | 89-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 425.31 | 829.69 | 3176.19 | 448.82 | 895.81 | 3347.96 |
| Standard deviation | 41.08 | 91.76 | 365.26 | 51.17 | 65.04 | 264.49 |
| CV (%) | 9.66 | 11.06 | 11.50 | 11.40 | 7.26 | 7.90 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
