| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This TGFBI Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TGFBI |
| Gene ID: |
7045 |
| Uniprot ID: |
BGH3_HUMAN |
| Specificity: |
This kit recognizes Human TGF Beta I in samples. No significant cross-reactivity or interference between Human TGF Beta I and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain. |
| Function | Plays a role in cell adhesion. May play a role in cell-collagen interactions. |
| Protein Name | Transforming Growth Factor-Beta-Induced Protein Ig-H3Beta Ig-H3Kerato-EpithelinRgd-Containing Collagen-Associated ProteinRgd-Cap |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixMay Be Associated Both With Microfibrils And With The Cell Surface |
| Alternative ELISA Names | Transforming Growth Factor-Beta-Induced Protein Ig-H3 ELISA kitBeta Ig-H3 ELISA kitKerato-Epithelin ELISA kitRgd-Containing Collagen-Associated Protein ELISA kitRgd-Cap ELISA kitTGFBI ELISA kitBIGH3 ELISA kit |
| Specificity | This kit recognizes Human TGFβI in samples. No significant cross-reactivity or interference between Human TGFβI and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-97 | 92 |
| EDTA plasma (n=8) | 93-105 | 98 |
| Cell culture media (n=8) | 88-102 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.09 | 4.11 | 14.96 | 2.13 | 4.18 | 14.94 |
| Standard deviation | 0.14 | 0.22 | 0.70 | 0.14 | 0.18 | 0.67 |
| CV (%) | 6.70 | 5.35 | 4.68 | 6.57 | 4.31 | 4.48 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
