| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human SMCP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
SMCP |
| Gene ID: |
4184 |
| Uniprot ID: |
MCSP_HUMAN |
| Specificity: |
This kit recognizes Human MCSP in samples. No significant cross-reactivity or interference between Human MCSP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Testis. Is selectively expressed in the spermatids of seminiferous tubules. |
| Function | Involved in sperm motility. Its absence is associated with genetic background dependent male infertility. Infertility may be due to reduced sperm motility in the female reproductive tract and inability to penetrate the oocyte zona pellucida. |
| Protein Name | Sperm Mitochondrial-Associated Cysteine-Rich Protein |
| Cellular Localisation | CytoplasmMitochondrion MembranePeripheral Membrane ProteinCytoplasmic SideBecomes Associated With The Spermatid Mitochondrion Capsule At Step 16 Of Spermatogenesis |
| Alternative CLIA Names | Sperm Mitochondrial-Associated Cysteine-Rich Protein CLIA kitSMCP CLIA kitMCS CLIA kitMCSP CLIA kit |
| Specificity | This kit recognizes Human MCSP in samples. No significant cross-reactivity or interference between Human MCSP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 99 |
| EDTA plasma(n=8) | 85-99 | 92 |
| Cell culture media(n=8) | 88-102 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.68 | 289.81 | 857.89 | 97.07 | 299.56 | 789.21 |
| Standard deviation | 10.04 | 34.31 | 71.55 | 8.51 | 27.83 | 64.79 |
| CV (%) | 9.50 | 11.84 | 8.34 | 8.77 | 9.29 | 8.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
