| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human SMAD1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
SMAD1 |
| Gene ID: |
4086 |
| Uniprot ID: |
SMAD1_HUMAN |
| Specificity: |
This kit recognizes Human Smad1/MADH1 in samples. No significant cross-reactivity or interference between Human Smad1/MADH1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitous. Highest expression seen in the heart and skeletal muscle. |
| Post Translational Modifications | Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Phosphorylation by ERK2 MAP kinase in response to EGF or HGF prevents SMAD1 nuclear accumulation and transcriptional activity in response to BMP. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. Dephosphorylation is inhibited by association with EGR1. Phosphorylation by CDK8/9 creates binding sites for YAP1, and subsequent phosphorylation by GSK3 switches off YAP1 binding and adds binding sites for SMURF1. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. Phospho-SMAD1 is ubiquitinated by CHIP leading to disruption of the SMAD1-SMAD4 complex. |
| Function | Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import. |
| Protein Name | Mothers Against Decapentaplegic Homolog 1Mad Homolog 1Mothers Against Dpp Homolog 1Jv4-1Mad-Related Protein 1Smad Family Member 1Smad 1Smad1Hsmad1Transforming Growth Factor-Beta-Signaling Protein 1Bsp-1 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-5689880Reactome: R-HSA-8941326Reactome: R-HSA-9733709 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4Co-Localizes With Lemd3 At The Nucleus Inner MembraneExported From The Nucleus To The Cytoplasm When Dephosphorylated |
| Alternative CLIA Names | Mothers Against Decapentaplegic Homolog 1 CLIA kitMad Homolog 1 CLIA kitMothers Against Dpp Homolog 1 CLIA kitJv4-1 CLIA kitMad-Related Protein 1 CLIA kitSmad Family Member 1 CLIA kitSmad 1 CLIA kitSmad1 CLIA kitHsmad1 CLIA kitTransforming Growth Factor-Beta-Signaling Protein 1 CLIA kitBsp-1 CLIA kitSMAD1 CLIA kitBSP1 CLIA kitMADH1 CLIA kitMADR1 CLIA kit |
| Specificity | This kit recognizes Human Smad1/MADH1 in samples. No significant cross-reactivity or interference between Human Smad1/MADH1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 93 |
| EDTA plasma(n=8) | 100-112 | 106 |
| Cell culture media(n=8) | 94-111 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.51 | 1.02 | 4.12 | 0.49 | 0.97 | 4.49 |
| Standard deviation | 0.06 | 0.11 | 0.30 | 0.06 | 0.10 | 0.29 |
| CV (%) | 11.76 | 10.78 | 7.28 | 12.24 | 10.31 | 6.46 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
