| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.051ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This SLC3A2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
SLC3A2 |
| Gene ID: |
6520 |
| Uniprot ID: |
4F2_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1. |
| Post Translational Modifications | N-glycosylated.N-glycosylation is crucial for trafficking and stability of SLC3A2 to the plasma membrane. Phosphorylation on Ser-406.Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions. |
| Function | Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters. Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids. Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane. SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA. SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis. (Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells. |
| Protein Name | Amino Acid Transporter Heavy Chain Slc3a24f2 Cell-Surface Antigen Heavy Chain4f2hc4f2 Heavy Chain AntigenLymphocyte Activation Antigen 4f2 Large SubunitSolute Carrier Family 3 Member 2Cd Antigen Cd98 |
| Database Links | Reactome: R-HSA-210991Reactome: R-HSA-352230Reactome: R-HSA-5660862Reactome: R-HSA-71240 |
| Cellular Localisation | Apical Cell MembraneCell MembraneSingle-Pass Type Ii Membrane ProteinCell JunctionLysosome MembraneMelanosomeBasolateral Cell MembraneLocalized At The Plasma Membrane When Associated With Slc7a5/Lat1 Or Slc7a8/Lat2Localized To The Apical Membrane Of Placental Syncytiotrophoblastic CellsRecruited To Lysosomes By Laptm4bIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocated Selectively At Cell-Cell Adhesion SitesColocalized With Slc7a8/Lat2 At The Basolateral Membrane Of Kidney Proximal Tubules And Small Intestine EpitheliaExpressed In Both Luminal And Abluminal Membranes Of Brain Capillary Endothelial Cells |
| Alternative ELISA Names | Amino Acid Transporter Heavy Chain Slc3a2 ELISA kit4f2 Cell-Surface Antigen Heavy Chain ELISA kit4f2hc ELISA kit4f2 Heavy Chain Antigen ELISA kitLymphocyte Activation Antigen 4f2 Large Subunit ELISA kitSolute Carrier Family 3 Member 2 ELISA kitCd Antigen Cd98 ELISA kitSLC3A2 ELISA kitMDU1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
