| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.116ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This SLC16A1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
SLC16A1 |
| Gene ID: |
6566 |
| Uniprot ID: |
MOT1_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Widely expressed. Detected in heart and in blood lymphocytes and monocytes (at protein level). |
| Post Translational Modifications | |
| Function | Bidirectional proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH. The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis. Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 309-Asp is essential for the conformational transition. |
| Protein Name | Monocarboxylate Transporter 1Mct 1Solute Carrier Family 16 Member 1 |
| Database Links | Reactome: R-HSA-210991Reactome: R-HSA-433692Reactome: R-HSA-5619070Reactome: R-HSA-70268Reactome: R-HSA-9749641 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneApical Cell MembraneExpression At The Cell Surface Requires The Ancillary Proteins Bsg And EmbBinds Preferentially To Bsg |
| Alternative ELISA Names | Monocarboxylate Transporter 1 ELISA kitMct 1 ELISA kitSolute Carrier Family 16 Member 1 ELISA kitSLC16A1 ELISA kitMCT1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
