| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human RPS6KB1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
RPS6KB1 |
| Gene ID: |
6198 |
| Uniprot ID: |
KS6B1_HUMAN |
| Specificity: |
This kit recognizes Human RPS6K Beta 1 in samples. No significant cross-reactivity or interference between Human RPS6K Beta 1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion. |
| Function | Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mechanistic target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition. |
| Protein Name | Ribosomal Protein S6 Kinase Beta-1S6k-Beta-1S6k170 Kda Ribosomal Protein S6 Kinase 1P70s6k1P70-S6k 1Ribosomal Protein S6 Kinase ISerine/Threonine-Protein Kinase 14aP70 Ribosomal S6 Kinase AlphaP70 S6 Kinase AlphaP70 S6k-AlphaP70 S6ka |
| Database Links | Reactome: R-HSA-166208 |
| Cellular Localisation | SynapseSynaptosomeMitochondrion Outer MembraneMitochondrionColocalizes With Uri1 At MitochondrionIsoform Alpha I: NucleusCytoplasmIsoform Alpha Ii: Cytoplasm |
| Alternative CLIA Names | Ribosomal Protein S6 Kinase Beta-1 CLIA kitS6k-Beta-1 CLIA kitS6k1 CLIA kit70 Kda Ribosomal Protein S6 Kinase 1 CLIA kitP70s6k1 CLIA kitP70-S6k 1 CLIA kitRibosomal Protein S6 Kinase I CLIA kitSerine/Threonine-Protein Kinase 14a CLIA kitP70 Ribosomal S6 Kinase Alpha CLIA kitP70 S6 Kinase Alpha CLIA kitP70 S6k-Alpha CLIA kitP70 S6ka CLIA kitRPS6KB1 CLIA kitSTK14A CLIA kit |
| Specificity | This kit recognizes Human RPS6Kβ1 in samples. No significant cross-reactivity or interference between Human RPS6Kβ1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-102 | 96 |
| EDTA plasma(n=8) | 97-108 | 102 |
| Cell culture media(n=8) | 89-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 110.09 | 293.02 | 771.96 | 112.10 | 296.47 | 736.90 |
| Standard deviation | 13.97 | 25.84 | 57.82 | 12.29 | 27.42 | 68.16 |
| CV (%) | 12.69 | 8.82 | 7.49 | 10.96 | 9.25 | 9.25 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
