| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human PYY kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PYY |
| Gene ID: |
5697 |
| Uniprot ID: |
PYY_HUMAN |
| Specificity: |
This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The peptide YY form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro) to generate peptide YY(3-36). |
| Function | This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. |
| Protein Name | Peptide YyPyyPyy-IPeptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36Pyy-Ii |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Peptide Yy CLIA kitPyy CLIA kitPyy-I CLIA kitPeptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 CLIA kitPyy-Ii CLIA kitPYY CLIA kit |
| Specificity | This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 101 |
| EDTA plasma(n=8) | 90-105 | 97 |
| Cell culture media(n=8) | 90-103 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 25.94 | 58.18 | 178.19 | 24.38 | 63.39 | 167.28 |
| Standard deviation | 3.16 | 6.85 | 17.62 | 2.63 | 6.20 | 17.11 |
| CV (%) | 12.18 | 11.77 | 9.89 | 10.79 | 9.78 | 10.23 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
