• Human PYY (Peptide YY) CLIA Kit (STJC0000564)

Human PYY (Peptide YY) CLIA Kit (STJC0000564)

SKU:
STJC0000564

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human PYY kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: PYY
Gene ID: 5697
Uniprot ID: PYY_HUMAN
Specificity: This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The peptide YY form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro) to generate peptide YY(3-36).
Function This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Protein Name Peptide Yy
Pyy
Pyy-I
Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36
Pyy-Ii
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative CLIA Names Peptide Yy CLIA kit
Pyy CLIA kit
Pyy-I CLIA kit
Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 CLIA kit
Pyy-Ii CLIA kit
PYY CLIA kit
Specificity This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 101
EDTA plasma(n=8) 90-105 97
Cell culture media(n=8) 90-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.94 58.18 178.19 24.38 63.39 167.28
Standard deviation 3.16 6.85 17.62 2.63 6.20 17.11
CV (%) 12.18 11.77 9.89 10.79 9.78 10.23