Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 4.69pg/mL |
Detection Limit: | 7.81~500pg/mL |
Short Description: | This human PYY kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | PYY |
Gene ID: | 5697 |
Uniprot ID: | PYY_HUMAN |
Specificity: | This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | The peptide YY form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro) to generate peptide YY(3-36). |
Function | This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. |
Protein Name | Peptide Yy Pyy Pyy-I Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 Pyy-Ii |
Database Links | Reactome: R-HSA-375276 Reactome: R-HSA-418594 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Peptide Yy CLIA kit Pyy CLIA kit Pyy-I CLIA kit Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 CLIA kit Pyy-Ii CLIA kit PYY CLIA kit |
Specificity | This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 95-108 | 101 |
EDTA plasma(n=8) | 90-105 | 97 |
Cell culture media(n=8) | 90-103 | 97 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 25.94 | 58.18 | 178.19 | 24.38 | 63.39 | 167.28 |
Standard deviation | 3.16 | 6.85 | 17.62 | 2.63 | 6.20 | 17.11 |
CV (%) | 12.18 | 11.77 | 9.89 | 10.79 | 9.78 | 10.23 |