| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This pERK1/2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | MAPK1 |
| Gene ID: | 5594 |
| Uniprot ID: | MK01_HUMAN |
| Specificity: | This kit recognizes Human pERK1/2 in samples. No significant cross-reactivity or interference between Human pERK1/2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2. Acts as a transcriptional repressor. Binds to a GCAAAGC consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. |
| Protein Name | Mitogen-Activated Protein Kinase 1Map Kinase 1Mapk 1Ert1Extracellular Signal-Regulated Kinase 2Erk-2Map Kinase Isoform P42P42-MapkMitogen-Activated Protein Kinase 2Map Kinase 2Mapk 2 |
| Database Links | Reactome: R-HSA-111995Reactome: R-HSA-112409Reactome: R-HSA-112411Reactome: R-HSA-1181150Reactome: R-HSA-1295596Reactome: R-HSA-1502540Reactome: R-HSA-162658Reactome: R-HSA-170968Reactome: R-HSA-198753Reactome: R-HSA-202670Reactome: R-HSA-2029482Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-2871796Reactome: R-HSA-3371453Reactome: R-HSA-375165Reactome: R-HSA-437239Reactome: R-HSA-444257Reactome: R-HSA-445144Reactome: R-HSA-450341Reactome: R-HSA-456926Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5663213Reactome: R-HSA-5668599Reactome: R-HSA-5673001Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5675221Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-6811558Reactome: R-HSA-74749Reactome: R-HSA-877300Reactome: R-HSA-879415Reactome: R-HSA-881907Reactome: R-HSA-8939211Reactome: R-HSA-8940973Reactome: R-HSA-8943724Reactome: R-HSA-9627069Reactome: R-HSA-9634635Reactome: R-HSA-9634638Reactome: R-HSA-9635465Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9652817Reactome: R-HSA-9656223Reactome: R-HSA-9664422Reactome: R-HSA-9725371Reactome: R-HSA-9732724Reactome: R-HSA-9768919Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleNucleusMicrotubule Organizing CenterCentrosomeMembraneCaveolaCell JunctionFocal AdhesionAssociated With The Spindle During Prometaphase And MetaphasePea15-Binding And Phosphorylated Dapk1 Promote Its Cytoplasmic RetentionPhosphorylation At Ser- 246 And Ser-248 As Well As Autophosphorylation At Thr-190 Promote Nuclear Localization |
| Alternative ELISA Names | Mitogen-Activated Protein Kinase 1 ELISA kitMap Kinase 1 ELISA kitMapk 1 ELISA kitErt1 ELISA kitExtracellular Signal-Regulated Kinase 2 ELISA kitErk-2 ELISA kitMap Kinase Isoform P42 ELISA kitP42-Mapk ELISA kitMitogen-Activated Protein Kinase 2 ELISA kitMap Kinase 2 ELISA kitMapk 2 ELISA kitMAPK1 ELISA kitERK2 ELISA kitPRKM1 ELISA kitPRKM2 ELISA kit |
| Specificity | This kit recognizes Human pERK1/2 in samples. No significant cross-reactivity or interference between Human pERK1/2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-107 | 100 |
| EDTA plasma (n=8) | 95-106 | 100 |
| Cell culture media (n=8) | 94-107 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 93.43 | 252.28 | 963.35 | 97.71 | 256.64 | 874.21 |
| Standard deviation | 5.10 | 14.56 | 36.13 | 6.08 | 10.60 | 43.36 |
| CV (%) | 5.46 | 5.77 | 3.75 | 6.22 | 4.13 | 4.96 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
