• Rat ERK2 (Extracellular Signal Regulated Kinase 2) Sandwich ELISA Kit Ready-To-Use (STJE0015079)

Rat ERK2 (Extracellular Signal Regulated Kinase 2) Sandwich ELISA Kit Ready-To-Use (STJE0015079)

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STJE0015079

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.061ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This ERK2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Mapk1
Gene ID: 116590
Uniprot ID: MK01_RAT
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Highest levels within the nervous system, expressed in different tissues, mostly in muscle, thymus and heart.
Post Translational Modifications Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Phosphorylated upon FLT3 and KIT signaling. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-185. Autophosphorylated on threonine and tyrosine residues in vitro, which correlates with a slow and low level of activation. Phosphorylation on Ser-27 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Dephosphorylated by DUSP1 and DUSP2 at Thr-183 and Tyr-185. ISGylated. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination.leading to activation. Deubiquitinated by CYLD.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2. Acts as a transcriptional repressor. Binds to a GCAAAGC consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Protein Name Mitogen-Activated Protein Kinase 1
Map Kinase 1
Mapk 1
Ert1
Extracellular Signal-Regulated Kinase 2
Erk-2
Map Kinase Isoform P42
P42-Mapk
Mitogen-Activated Protein Kinase 2
Map Kinase 2
Mapk 2
Database Links Reactome: R-RNO-111995
Reactome: -RNO-112409
Reactome: -RNO-112411
Reactome: -RNO-1181150
Reactome: -RNO-1295596
Reactome: -RNO-1502540
Reactome: -RNO-162658
Reactome: -RNO-170968
Reactome: -RNO-198753
Reactome: -RNO-202670
Reactome: -RNO-2029482
Reactome: -RNO-2173795
Reactome: -RNO-2173796
Reactome: -RNO-2559580
Reactome: -RNO-2559582
Reactome: -RNO-2559585
Reactome: -RNO-2871796
Reactome: -RNO-3371453
Reactome: -RNO-375165
Reactome: -RNO-437239
Reactome: -RNO-445144
Reactome: -RNO-450341
Reactome: -RNO-456926
Reactome: -RNO-5654726
Reactome: -RNO-5654727
Reactome: -RNO-5654732
Reactome: -RNO-5654733
Reactome: -RNO-5663213
Reactome: -RNO-5668599
Reactome: -RNO-5673001
Reactome: -RNO-5674135
Reactome: -RNO-5674499
Reactome: -RNO-5675221
Reactome: -RNO-6798695
Reactome: -RNO-6811558
Reactome: -RNO-74749
Reactome: -RNO-877300
Reactome: -RNO-881907
Reactome: -RNO-8939211
Reactome: -RNO-9627069
Reactome: -RNO-9634635
Reactome: -RNO-9634638
Reactome: -RNO-9732724
Reactome: -RNO-982772
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Nucleus
Microtubule Organizing Center
Centrosome
Membrane
Caveola
Cell Junction
Focal Adhesion
Associated With The Spindle During Prometaphase And Metaphase
Pea15-Binding And Phosphorylated Dapk1 Promote Its Cytoplasmic Retention
Phosphorylation At Ser- 244 And Ser-246 As Well As Autophosphorylation At Thr-188 Promote Nuclear Localization
Alternative ELISA Names Mitogen-Activated Protein Kinase 1 ELISA kit
Map Kinase 1 ELISA kit
Mapk 1 ELISA kit
Ert1 ELISA kit
Extracellular Signal-Regulated Kinase 2 ELISA kit
Erk-2 ELISA kit
Map Kinase Isoform P42 ELISA kit
P42-Mapk ELISA kit
Mitogen-Activated Protein Kinase 2 ELISA kit
Map Kinase 2 ELISA kit
Mapk 2 ELISA kit
Mapk1 ELISA kit
Erk2 ELISA kit
Mapk ELISA kit
Prkm1 ELISA kit
output

Information sourced from Uniprot.org

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