| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This NPPA Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 2.5h |
| Detection: | Colormetric |
| Gene Symbol: | NPPA |
| Gene ID: | 4878 |
| Uniprot ID: | ANF_HUMAN |
| Specificity: | This kit recognizes Human ANP in samples. No significant cross-reactivity or interference between Human ANP and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | The precursor molecule is proteolytically cleaved by CORIN at Arg-123 to produce atrial natriuretic peptide. Undergoes further proteolytic cleavage by unknown proteases to give rise to long-acting natriuretic peptide, vessel dilator and kaliuretic peptide. Additional processing gives rise to the auriculin and atriopeptin peptides. In the kidneys, alternative processing by an unknown protease results in the peptide urodilatin. Atrial natriuretic peptide: Cleavage by MME initiates degradation of the factor and thereby regulates its activity. Degraded by IDE (in vitro). During IDE degradation, the resulting products can temporarily stimulate NPR2 to produce cGMP, before the fragments are completely degraded and inactivated by IDE (in vitro). Urodilatin: Degraded by IDE. Urodilatin: Phosphorylation on Ser-129 decreases vasorelaxant activity. |
| Function | Atrial natriuretic peptide: Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses. Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance. Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts. Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension. In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis. This includes up-regulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue. Binds the clearance receptor NPR3 which removes the hormone from circulation. Long-acting natriuretic peptide: May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, vasodilation, and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting.according to a report, in vivo it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by atrial natriuretic peptide and vessel dilator. Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption. Vessel dilator: May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, and vasodilation. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting.according to a report it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by the atrial natriuretic and long-acting natriuretic peptides. Possibly functions in protein excretion in urine by maintaining the integrity of the proximal tubules and enhancing protein excretion by decreasing proximal tubular protein reabsorption. Kaliuretic peptide: May have a role in cardio-renal homeostasis through regulation of diuresis and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. May have a role in potassium excretion but not sodium excretion (natriuresis). Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption. Urodilatin: Hormone produced in the kidneys that appears to be important for maintaining cardio-renal homeostasis. Mediates vasodilation, natriuresis and diuresis primarily in the renal system, in order to maintain the extracellular fluid volume and control the fluid-electrolyte balance. Specifically binds and stimulates cGMP production by renal transmembrane receptors, likely NPR1. Urodilatin not ANP, may be the natriuretic peptide responsible for the regulation of sodium and water homeostasis in the kidney. Auriculin-D: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-B: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-A: May have a role in cardio-renal homeostasis through regulation of regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, vasodilates intestinal smooth muscle but not smooth muscle strips. Atriopeptin-2: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal and vascular smooth muscle strips. Atriopeptin-1: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal smooth muscle but not vascular smooth muscle strips. |
| Protein Name | Natriuretic Peptides AAtrial Natriuretic Factor ProhormoneProanfAtrial Natriuretic Peptide ProhormonePreproanpProanpAtriopeptigenCardiodilatinCddPreprocdd-Anf Cleaved Into - Long-Acting Natriuretic PeptideLanpLong-Acting Natriuretic HormoneLanhPro Atrial Natriuretic Factor 1-30Proanf 1-30Pro Atrial Natriuretic Peptide 1-30Proanp 1-30 - Vessel DilatorVsdlPro Atrial Natriuretic Factor 31-67Proanf 31-67Pro Atrial Natriuretic Peptide 31-67Proanp 31-67 - Kaliuretic PeptideKpPro Atrial Natriuretic Factor 79-98Proanf 79-98Pro Atrial Natriuretic Peptide 79-98Proanp 79-98 - UrodilatinUroCdd 95-126Cdd-Anp95-126Pro Atrial Natriuretic Peptide 95-126Proanp 95-126 - Auriculin-CAtrial Natriuretic Factor 1-33Anf 1-33 - Auriculin-DAtrial Natriuretic Factor 3-33Anf 3-33 - Atrial Natriuretic PeptideAnpAlpha-Atrial Natriuretic PeptideAlpha-HanpAtrial Natriuretic FactorAnfCdd-AnfCdd-Anp99-126CardionatrinPro Atrial Natriuretic Factor 99-126Proanf 99-126 - Auriculin-BAtrial Natriuretic Factor 8-33Anf 8-33 - Auriculin-A - Atriopeptin-1Atriopeptin I - Atriopeptin-2Atriopeptin Ii - Atriopeptin-3Atriopeptin Iii |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-5578768Reactome: R-HSA-977225 |
| Cellular Localisation | Long-Acting Natriuretic Peptide: SecretedDetected In BloodVessel Dilator: SecretedKaliuretic Peptide: SecretedUrodilatin: SecretedDetected In UrineNot Detected In BloodIncreased ElectrolytesOsmolality And Intracellular Camp Levels Increase Peptide Secretion/ExcretionAtrial Natriuretic Peptide: SecretedPerikaryonCell ProjectionDetected In Urine In One StudyHoweverIn Another StudyWas Not Detected In UrineDetected In Cytoplasmic Bodies And Neuronal Processes Of Pyramidal Neurons (Layers Ii-Vi)Increased Secretion In Response To The Vasopressin AvpLikely To Be Secreted In Response To An Increase In Atrial Pressure Or Atrial StretchIn Kidney CellsSecretion Increases In Response To Activated Guanylyl Cyclases And Increased Intracellular Camp LevelsPlasma Levels Increase 15 Minutes After A High-Salt MealAnd Decrease Back To Normal Plasma Levels 1 Hr LaterAtriopeptin-3: SecretedSlight Increase In Secretion In Response To The Vasopressin Avp |
| Alternative ELISA Names | Natriuretic Peptides A ELISA kitAtrial Natriuretic Factor Prohormone ELISA kitProanf ELISA kitAtrial Natriuretic Peptide Prohormone ELISA kitPreproanp ELISA kitProanp ELISA kitAtriopeptigen ELISA kitCardiodilatin ELISA kitCdd ELISA kitPreprocdd-Anf Cleaved Into - Long-Acting Natriuretic Peptide ELISA kitLanp ELISA kitLong-Acting Natriuretic Hormone ELISA kitLanh ELISA kitPro Atrial Natriuretic Factor 1-30 ELISA kitProanf 1-30 ELISA kitPro Atrial Natriuretic Peptide 1-30 ELISA kitProanp 1-30 - Vessel Dilator ELISA kitVsdl ELISA kitPro Atrial Natriuretic Factor 31-67 ELISA kitProanf 31-67 ELISA kitPro Atrial Natriuretic Peptide 31-67 ELISA kitProanp 31-67 - Kaliuretic Peptide ELISA kitKp ELISA kitPro Atrial Natriuretic Factor 79-98 ELISA kitProanf 79-98 ELISA kitPro Atrial Natriuretic Peptide 79-98 ELISA kitProanp 79-98 - Urodilatin ELISA kitUro ELISA kitCdd 95-126 ELISA kitCdd-Anp ELISA kit95-126 ELISA kitPro Atrial Natriuretic Peptide 95-126 ELISA kitProanp 95-126 - Auriculin-C ELISA kitAtrial Natriuretic Factor 1-33 ELISA kitAnf 1-33 - Auriculin-D ELISA kitAtrial Natriuretic Factor 3-33 ELISA kitAnf 3-33 - Atrial Natriuretic Peptide ELISA kitAnp ELISA kitAlpha-Atrial Natriuretic Peptide ELISA kitAlpha-Hanp ELISA kitAtrial Natriuretic Factor ELISA kitAnf ELISA kitCdd-Anf ELISA kitCdd-Anp ELISA kit99-126 ELISA kitCardionatrin ELISA kitPro Atrial Natriuretic Factor 99-126 ELISA kitProanf 99-126 - Auriculin-B ELISA kitAtrial Natriuretic Factor 8-33 ELISA kitAnf 8-33 - Auriculin-A - Atriopeptin-1 ELISA kitAtriopeptin I - Atriopeptin-2 ELISA kitAtriopeptin Ii - Atriopeptin-3 ELISA kitAtriopeptin Iii ELISA kitNPPA ELISA kitANP ELISA kitPND ELISA kit |
| Specificity | This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-100 | 92 |
| EDTA plasma(n=8) | 94-104 | 99 |
| Cell culture media(n=8) | 92-107 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 26.80 | 74.50 | 197.10 | 29.00 | 80.30 | 208.60 |
| Standard deviation | 1.70 | 3.90 | 8.90 | 1.70 | 4.60 | 11.50 |
| CV (%) | 6.34 | 5.23 | 4.52 | 5.86 | 5.73 | 5.51 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
