• Mouse ANP (Atrial Natriuretic Peptide) Competitive ELISA (STJE0004309)

Mouse ANP (Atrial Natriuretic Peptide) Competitive ELISA (STJE0004309)

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STJE0004309

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This ANP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: Nppa
Gene ID: 230899
Uniprot ID: ANF_MOUSE
Specificity: This kit recognizes Mouse ANP in samples. No significant cross-reactivity or interference between Mouse ANP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Atrial natriuretic peptide: Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses. Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance. Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts. Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension. In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis. This includes up-regulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue. Binds the clearance receptor NPR3 which removes the hormone from circulation. Long-acting natriuretic peptide: May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, vasodilation, and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting.according to a report, in vivo it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by atrial natriuretic peptide and vessel dilator. Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption. Vessel dilator: May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, and vasodilation. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting.according to a report it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by the atrial natriuretic and long-acting natriuretic peptides. Possibly functions in protein excretion in urine by maintaining the integrity of the proximal tubules and enhancing protein excretion by decreasing proximal tubular protein reabsorption. Kaliuretic peptide: May have a role in cardio-renal homeostasis through regulation of diuresis and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. May have a role in potassium excretion but not sodium excretion (natriuresis). Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption. Urodilatin: Hormone produced in the kidneys that appears to be important for maintaining cardio-renal homeostasis. Mediates vasodilation, natriuresis and diuresis primarily in the renal system, in order to maintain the extracellular fluid volume and control the fluid-electrolyte balance. Specifically binds and stimulates cGMP production by renal transmembrane receptors, likely NPR1. Urodilatin not ANP, may be the natriuretic peptide responsible for the regulation of sodium and water homeostasis in the kidney. Auriculin-D: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-B: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-A: May have a role in cardio-renal homeostasis through regulation of regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, vasodilates intestinal smooth muscle but not smooth muscle strips. Atriopeptin-2: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal and vascular smooth muscle strips. Atriopeptin-1: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal smooth muscle but not vascular smooth muscle strips.
Protein Name Natriuretic Peptides A
Atrial Natriuretic Factor Prohormone
Preproanf
Proanf
Atrial Natriuretic Peptide Prohormone
Preproanp
Proanp
Atriopeptigen
Cardiodilatin
Cdd
Preprocdd-Anf Cleaved Into - Long-Acting Natriuretic Peptide
Lanp
Long-Acting Natriuretic Hormone
Lanh
Pro Atrial Natriuretic Factor 1-30
Proanf 1-30
Pro Atrial Natriuretic Peptide 1-30
Proanp 1-30 - Vessel Dilator
Vsdl
Pro Atrial Natriuretic Factor 31-67
Proanf 31-67
Pro Atrial Natriuretic Peptide 31-67
Proanp 31-67 - Kaliuretic Peptide
Kp
Pro Atrial Natriuretic Factor 79-98
Proanf 79-98
Pro Atrial Natriuretic Peptide 79-98
Proanp 79-98 - Urodilatin
Uro
Cdd 95-126
Cdd-Anp
95-126
Pro Atrial Natriuretic Peptide 95-126
Proanp 95-126 - Auriculin-C
Atrial Natriuretic Factor 1-33
Anf 1-33 - Auriculin-D
Atrial Natriuretic Factor 3-33
Anf 3-33 - Atrial Natriuretic Peptide
Anp
Alpha-Atrial Natriuretic Peptide
Alpha-Hanp
Atrial Natriuretic Factor
Anf
Cdd-Anf
Cdd-Anp
99-126
Cardionatrin
Pro Atrial Natriuretic Factor 99-126
Proanf 99-126 - Auriculin-B
Atrial Natriuretic Factor 8-33
Anf 8-33 - Auriculin-A - Atriopeptin-1
Atriopeptin I - Atriopeptin-2
Atriopeptin Ii - Atriopeptin-3
Atriopeptin Iii
Database Links Reactome: R-MMU-5578768
Cellular Localisation Long-Acting Natriuretic Peptide: Secreted
Detected In Blood
Vessel Dilator: Secreted
Kaliuretic Peptide: Secreted
Urodilatin: Secreted
Detected In Urine
Not Detected In Blood
Increased Electrolytes
Osmolality And Intracellular Camp Levels Increase Peptide Secretion/Excretion
Atrial Natriuretic Peptide: Secreted
Perikaryon
Cell Projection
Detected In Urine In One Study
However
In Another Study
Was Not Detected In Urine
Detected In Cytoplasmic Bodies And Neuronal Processes Of Pyramidal Neurons (Layers Ii-Vi)
Increased Secretion In Response To The Vasopressin Avp
Likely To Be Secreted In Response To An Increase In Atrial Pressure Or Atrial Stretch
In Kidney Cells
Secretion Increases In Response To Activated Guanylyl Cyclases And Increased Intracellular Camp Levels
Plasma Levels Increase 15 Minutes After A High-Salt Meal
And Decrease Back To Normal Plasma Levels 1 Hr Later
Atriopeptin-3: Secreted
Slight Increase In Secretion In Response To The Vasopressin Avp
Alternative ELISA Names Natriuretic Peptides A ELISA kit
Atrial Natriuretic Factor Prohormone ELISA kit
Preproanf ELISA kit
Proanf ELISA kit
Atrial Natriuretic Peptide Prohormone ELISA kit
Preproanp ELISA kit
Proanp ELISA kit
Atriopeptigen ELISA kit
Cardiodilatin ELISA kit
Cdd ELISA kit
Preprocdd-Anf Cleaved Into - Long-Acting Natriuretic Peptide ELISA kit
Lanp ELISA kit
Long-Acting Natriuretic Hormone ELISA kit
Lanh ELISA kit
Pro Atrial Natriuretic Factor 1-30 ELISA kit
Proanf 1-30 ELISA kit
Pro Atrial Natriuretic Peptide 1-30 ELISA kit
Proanp 1-30 - Vessel Dilator ELISA kit
Vsdl ELISA kit
Pro Atrial Natriuretic Factor 31-67 ELISA kit
Proanf 31-67 ELISA kit
Pro Atrial Natriuretic Peptide 31-67 ELISA kit
Proanp 31-67 - Kaliuretic Peptide ELISA kit
Kp ELISA kit
Pro Atrial Natriuretic Factor 79-98 ELISA kit
Proanf 79-98 ELISA kit
Pro Atrial Natriuretic Peptide 79-98 ELISA kit
Proanp 79-98 - Urodilatin ELISA kit
Uro ELISA kit
Cdd 95-126 ELISA kit
Cdd-Anp ELISA kit
95-126 ELISA kit
Pro Atrial Natriuretic Peptide 95-126 ELISA kit
Proanp 95-126 - Auriculin-C ELISA kit
Atrial Natriuretic Factor 1-33 ELISA kit
Anf 1-33 - Auriculin-D ELISA kit
Atrial Natriuretic Factor 3-33 ELISA kit
Anf 3-33 - Atrial Natriuretic Peptide ELISA kit
Anp ELISA kit
Alpha-Atrial Natriuretic Peptide ELISA kit
Alpha-Hanp ELISA kit
Atrial Natriuretic Factor ELISA kit
Anf ELISA kit
Cdd-Anf ELISA kit
Cdd-Anp ELISA kit
99-126 ELISA kit
Cardionatrin ELISA kit
Pro Atrial Natriuretic Factor 99-126 ELISA kit
Proanf 99-126 - Auriculin-B ELISA kit
Atrial Natriuretic Factor 8-33 ELISA kit
Anf 8-33 - Auriculin-A - Atriopeptin-1 ELISA kit
Atriopeptin I - Atriopeptin-2 ELISA kit
Atriopeptin Ii - Atriopeptin-3 ELISA kit
Atriopeptin Iii ELISA kit
Nppa ELISA kit
Pnd ELISA kit
Specificity This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-109 101
EDTA plasma(n=8) 93-105 98
Cell culture media(n=8) 84-99 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.50 157.20 342.30 43.00 163.10 362.80
Standard deviation 3.00 7.70 13.30 2.60 7.20 12.70
CV (%) 6.32 4.90 3.89 6.05 4.41 3.50
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance