| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This Nppb Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Nppb |
| Gene ID: |
25105 |
| Uniprot ID: |
ANFB_RAT |
| Specificity: |
This kit recognizes Rat BNP in samples. No significant cross-reactivity or interference between Rat BNP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The precursor molecule is proteolytically cleaved by the endoprotease Furin to produce brain natriuretic peptide 45. May undergo further proteolytic cleavage by various proteases such as DPP4, MME and possibly FAP, to give rise to a variety of shorter peptides. May be cleaved at Ser-91 by the prolyl endopeptidase FAP (seprase) activity (in vitro). May be degraded by IDE. During IDE degradation, the resulting products initially increase the activation of NPR1 and can also stimulate NPR2 to produce cGMP before the fragments are completely degraded and inactivated by IDE (in vitro). |
| Function | Brain natriuretic peptide 45: Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis. |
| Protein Name | Natriuretic Peptides BBrain Natriuretic Factor ProhormonePreprobnpProbnpGamma-Brain Natriuretic PeptideIso-Anp Cleaved Into - Brain Natriuretic Peptide 45Bnp-455 Kda Cardiac Natriuretic PeptideBrain Natriuretic PeptideBnp |
| Cellular Localisation | Brain Natriuretic Peptide 45: Secreted |
| Alternative ELISA Names | Natriuretic Peptides B ELISA kitBrain Natriuretic Factor Prohormone ELISA kitPreprobnp ELISA kitProbnp ELISA kitGamma-Brain Natriuretic Peptide ELISA kitIso-Anp Cleaved Into - Brain Natriuretic Peptide 45 ELISA kitBnp-45 ELISA kit5 Kda Cardiac Natriuretic Peptide ELISA kitBrain Natriuretic Peptide ELISA kitBnp ELISA kitNppb ELISA kit |
| Specificity | This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 101 |
| EDTA plasma(n=8) | 84-97 | 91 |
| Cell culture media(n=8) | 86-98 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 96.20 | 313.10 | 672.80 | 97.20 | 338.80 | 634.50 |
| Standard deviation | 5.90 | 14.70 | 32.30 | 6.60 | 17.30 | 29.20 |
| CV (%) | 6.13 | 4.69 | 4.80 | 6.79 | 5.11 | 4.60 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
