Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 46.88pg/mL |
Detection Limit: | 78.13~5000pg/mL |
Short Description: | This NOS2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | NOS2 |
Gene ID: | 4843 |
Uniprot ID: | NOS2_HUMAN |
Specificity: | This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
Protein Name | Nitric Oxide Synthase - Inducible Hepatocyte Nos Hep-Nos Inducible No Synthase Inducible Nos Inos Nos Type Ii Peptidyl-Cysteine S-Nitrosylase Nos2 |
Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-6785807 Reactome: R-HSA-9033241 Reactome: R-HSA-9636249 |
Cellular Localisation | Cytoplasm Cytosol Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4 Exhibits A More Diffuse Cytosolic Localization |
Alternative ELISA Names | Nitric Oxide Synthase - Inducible ELISA kit Hepatocyte Nos ELISA kit Hep-Nos ELISA kit Inducible No Synthase ELISA kit Inducible Nos ELISA kit Inos ELISA kit Nos Type Ii ELISA kit Peptidyl-Cysteine S-Nitrosylase Nos2 ELISA kit NOS2 ELISA kit NOS2A ELISA kit |
Specificity | This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 95-108 | 101 |
EDTA plasma (n=8) | 94-107 | 99 |
Cell culture media (n=8) | 85-95 | 90 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 276.20 | 441.40 | 2054.00 | 265.80 | 481.50 | 2256.90 |
Standard deviation | 14.10 | 24.70 | 71.90 | 17.30 | 26.50 | 92.50 |
CV (%) | 5.10 | 5.60 | 3.50 | 6.51 | 5.50 | 4.10 |