• Human NOS2 (Nitric oxide synthase, inducible) Sandwich ELISA (STJE0002457)

Human NOS2 (Nitric oxide synthase, inducible) Sandwich ELISA (STJE0002457)

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STJE0002457

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This NOS2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: NOS2
Gene ID: 4843
Uniprot ID: NOS2_HUMAN
Specificity: This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
Protein Name Nitric Oxide Synthase - Inducible
Hepatocyte Nos
Hep-Nos
Inducible No Synthase
Inducible Nos
Inos
Nos Type Ii
Peptidyl-Cysteine S-Nitrosylase Nos2
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-392154
Reactome: R-HSA-6785807
Reactome: R-HSA-9033241
Reactome: R-HSA-9636249
Cellular Localisation Cytoplasm
Cytosol
Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4
Exhibits A More Diffuse Cytosolic Localization
Alternative ELISA Names Nitric Oxide Synthase - Inducible ELISA kit
Hepatocyte Nos ELISA kit
Hep-Nos ELISA kit
Inducible No Synthase ELISA kit
Inducible Nos ELISA kit
Inos ELISA kit
Nos Type Ii ELISA kit
Peptidyl-Cysteine S-Nitrosylase Nos2 ELISA kit
NOS2 ELISA kit
NOS2A ELISA kit
Specificity This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 101
EDTA plasma (n=8) 94-107 99
Cell culture media (n=8) 85-95 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 276.20 441.40 2054.00 265.80 481.50 2256.90
Standard deviation 14.10 24.70 71.90 17.30 26.50 92.50
CV (%) 5.10 5.60 3.50 6.51 5.50 4.10