| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This Nos2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Nos2 |
| Gene ID: |
18126 |
| Uniprot ID: |
NOS2_MOUSE |
| Specificity: |
This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - InducibleInducible No SynthaseInducible NosInosMacrophage NosMac-NosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-MMU-1222556Reactome: -MMU-392154Reactome: -MMU-9033241 |
| Cellular Localisation | CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization |
| Alternative ELISA Names | Nitric Oxide Synthase - Inducible ELISA kitInducible No Synthase ELISA kitInducible Nos ELISA kitInos ELISA kitMacrophage Nos ELISA kitMac-Nos ELISA kitNos Type Ii ELISA kitPeptidyl-Cysteine S-Nitrosylase Nos2 ELISA kitNos2 ELISA kitInosl ELISA kit |
| Specificity | This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-103 | 96 |
| EDTA plasma (n=8) | 89-102 | 95 |
| Cell culture media (n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.10 | 2.90 | 9.90 | 1.00 | 3.00 | 10.50 |
| Standard deviation | 0.10 | 0.20 | 0.30 | 0.10 | 0.20 | 0.50 |
| CV (%) | 9.09 | 6.90 | 3.03 | 10.00 | 6.67 | 4.76 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
