| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
3.75pg/mL |
| Detection Limit: |
6.25~400pg/mL |
| Short Description: |
This human KNG1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
KNG1 |
| Gene ID: |
3827 |
| Uniprot ID: |
KNG1_HUMAN |
| Specificity: |
This kit recognizes Human KNG1 in samples. No significant cross-reactivity or interference between Human KNG1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors. |
| Post Translational Modifications | Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus. Bradykinin is released from kininogen by plasma kallikrein. Hydroxylation of Pro-383 occurs prior to the release of bradykinin. Phosphorylated by FAM20C in the extracellular medium. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. (Microbial infection) Bradykinin is generated upon proteolytic cleavage by S.pyogenes SpeB to produce hypotension during septic shock. |
| Function | Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action). |
| Protein Name | Kininogen-1Alpha-2-Thiol Proteinase InhibitorFitzgerald FactorHigh Molecular Weight KininogenHmwkWilliams-Fitzgerald-Flaujeac Factor Cleaved Into - Kininogen-1 Heavy Chain - T-KininIle-Ser-Bradykinin - BradykininKallidin I - Lysyl-BradykininKallidin Ii - Kininogen-1 Light Chain - Low Molecular Weight Growth-Promoting Factor |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140837Reactome: R-HSA-375276Reactome: R-HSA-381426Reactome: R-HSA-416476Reactome: R-HSA-418594Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative CLIA Names | Kininogen-1 CLIA kitAlpha-2-Thiol Proteinase Inhibitor CLIA kitFitzgerald Factor CLIA kitHigh Molecular Weight Kininogen CLIA kitHmwk CLIA kitWilliams-Fitzgerald-Flaujeac Factor Cleaved Into - Kininogen-1 Heavy Chain - T-Kinin CLIA kitIle-Ser-Bradykinin - Bradykinin CLIA kitKallidin I - Lysyl-Bradykinin CLIA kitKallidin Ii - Kininogen-1 Light Chain - Low Molecular Weight Growth-Promoting Factor CLIA kitKNG1 CLIA kitBDK CLIA kitKNG CLIA kit |
| Specificity | This kit recognizes Human KNG1 in samples. No significant cross-reactivity or interference between Human KNG1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-98 | 91 |
| EDTA plasma(n=8) | 102-115 | 107 |
| Cell culture media(n=8) | 87-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 20.95 | 47.20 | 149.88 | 21.51 | 43.24 | 153.86 |
| Standard deviation | 2.52 | 5.49 | 9.17 | 2.27 | 3.12 | 12.59 |
| CV (%) | 12.03 | 11.63 | 6.12 | 10.55 | 7.22 | 8.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
