| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human IL-12A-C-Flag protein was developed from hek293 cells and has a target region of C-Flag. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Endotoxin: |
Please contact us for more information. |
| Immunoreactivity: |
Measured in a cell proliferation assay using anti-CD3 antibody activated Jurkat Human acute T lymphocyte leukemia cells. The ED50 for this effect is typically 0.075-0.3 ng/mL. |
| Gene Symbol: |
IL12A |
| Gene ID: |
3592 |
| Uniprot ID: |
IL12A_HUMAN |
| Immunogen Region: |
Arg23-Ser219 |
| Immunogen: |
Recombinant Human IL-12A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_000873.2) fused with a Flag tag at the C-terminus. |
| Function | Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites. |
| Protein Name | Interleukin-12 Subunit AlphaIl-12aCytotoxic Lymphocyte Maturation Factor 35 Kda SubunitClmf P35Il-12 Subunit P35Nk Cell Stimulatory Factor Chain 1Nksf1 |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8984722Reactome: R-HSA-9020591 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-12 Subunit Alpha proteinIl-12a proteinCytotoxic Lymphocyte Maturation Factor 35 Kda Subunit proteinClmf P35 proteinIl-12 Subunit P35 proteinNk Cell Stimulatory Factor Chain 1 proteinNksf1 proteinIL12A proteinNKSF1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
