| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This IGFBP-7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IGFBP7 |
| Gene ID: |
3490 |
| Uniprot ID: |
IBP7_HUMAN |
| Specificity: |
This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. |
| Function | Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 7Ibp-7Igf-Binding Protein 7Igfbp-7Igfbp-Rp1Mac25 ProteinPgi2-Stimulating FactorProstacyclin-Stimulating FactorTumor-Derived Adhesion FactorTaf |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor-Binding Protein 7 ELISA kitIbp-7 ELISA kitIgf-Binding Protein 7 ELISA kitIgfbp-7 ELISA kitIgfbp-Rp1 ELISA kitMac25 Protein ELISA kitPgi2-Stimulating Factor ELISA kitProstacyclin-Stimulating Factor ELISA kitTumor-Derived Adhesion Factor ELISA kitTaf ELISA kitIGFBP7 ELISA kitMAC25 ELISA kitPSF ELISA kit |
| Specificity | This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-96 | 91 |
| EDTA plasma (n=8) | 96-107 | 101 |
| Cell culture media (n=8) | 90-103 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 91.33 | 264.92 | 952.33 | 91.69 | 263.98 | 1011.09 |
| Standard deviation | 6.17 | 13.27 | 45.14 | 6.32 | 10.59 | 36.20 |
| CV (%) | 6.76 | 5.01 | 4.74 | 6.89 | 4.01 | 3.58 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
