| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
11.32 pg/mL |
| Detection Limit: |
31.25-2000 pg/mL |
| Short Description: |
This IGFBP-6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IGFBP6 |
| Gene ID: |
3489 |
| Uniprot ID: |
IBP6_HUMAN |
| Specificity: |
This kit recognizes Human IGFBP-6 in samples. No significant cross-reactivity or interference between Human IGFBP-6 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Post Translational Modifications | O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 6Ibp-6Igf-Binding Protein 6Igfbp-6 |
| Database Links | Reactome: R-HSA-381426 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor-Binding Protein 6 ELISA kitIbp-6 ELISA kitIgf-Binding Protein 6 ELISA kitIgfbp-6 ELISA kitIGFBP6 ELISA kitIBP6 ELISA kit |
| Specificity | This kit recognizes Human IGFBP-6 in samples. No significant cross-reactivity or interference between Human IGFBP-6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-99 | 90 |
| EDTA plasma (n=8) | 87-101 | 93 |
| Cell culture media (n=8) | 85-96 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.42 | 314.69 | 999.02 | 105.86 | 314.06 | 1075.02 |
| Standard deviation | 5.77 | 14.38 | 52.85 | 6.34 | 17.90 | 55.69 |
| CV (%) | 5.58 | 4.57 | 5.29 | 5.99 | 5.70 | 5.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
