| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human GRN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GRN |
| Gene ID: |
2896 |
| Uniprot ID: |
GRN_HUMAN |
| Specificity: |
This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. |
| Post Translational Modifications | Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome. |
| Function | Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. |
| Protein Name | ProgranulinPgrnAcrograninEpithelin PrecursorGlycoprotein Of 88 KdaGp88Glycoprotein 88Granulin PrecursorPc Cell-Derived Growth FactorPcdgfProepithelinPepi Cleaved Into - Paragranulin - Granulin-1Granulin G - Granulin-2Granulin F - Granulin-3Epithelin-2Granulin B - Granulin-4Epithelin-1Granulin A - Granulin-5Granulin C - Granulin-6Granulin D - Granulin-7Granulin E |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedLysosomeEndocytosed By Sort1 And Delivred To LysosomesTargeted To Lysosome By Psap Via M6pr And Lrp1In Both Biosynthetic And Endocytic PathwaysCo-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome |
| Alternative CLIA Names | Progranulin CLIA kitPgrn CLIA kitAcrogranin CLIA kitEpithelin Precursor CLIA kitGlycoprotein Of 88 Kda CLIA kitGp88 CLIA kitGlycoprotein 88 CLIA kitGranulin Precursor CLIA kitPc Cell-Derived Growth Factor CLIA kitPcdgf CLIA kitProepithelin CLIA kitPepi Cleaved Into - Paragranulin - Granulin-1 CLIA kitGranulin G - Granulin-2 CLIA kitGranulin F - Granulin-3 CLIA kitEpithelin-2 CLIA kitGranulin B - Granulin-4 CLIA kitEpithelin-1 CLIA kitGranulin A - Granulin-5 CLIA kitGranulin C - Granulin-6 CLIA kitGranulin D - Granulin-7 CLIA kitGranulin E CLIA kitGRN CLIA kit |
| Specificity | This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-117 | 107 |
| EDTA plasma(n=8) | 94-105 | 100 |
| Cell culture media(n=8) | 93-106 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.95 | 1.85 | 7.92 | 0.94 | 1.98 | 8.59 |
| Standard deviation | 0.08 | 0.18 | 0.71 | 0.10 | 0.19 | 0.73 |
| CV (%) | 8.42 | 9.73 | 8.96 | 10.64 | 9.60 | 8.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
