• Human GRN (Progranulin) Sandwich ELISA (STJE0003076)
  • Human GRN (Progranulin) Sandwich ELISA (STJE0003076)

Human GRN (Progranulin) Sandwich ELISA (STJE0003076)

SKU:
STJE0003076

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This GRN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GRN
Gene ID: 2896
Uniprot ID: GRN_HUMAN
Specificity: This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome.
Function Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
Protein Name Progranulin
Pgrn
Acrogranin
Epithelin Precursor
Glycoprotein Of 88 Kda
Gp88
Glycoprotein 88
Granulin Precursor
Pc Cell-Derived Growth Factor
Pcdgf
Proepithelin
Pepi Cleaved Into - Paragranulin - Granulin-1
Granulin G - Granulin-2
Granulin F - Granulin-3
Epithelin-2
Granulin B - Granulin-4
Epithelin-1
Granulin A - Granulin-5
Granulin C - Granulin-6
Granulin D - Granulin-7
Granulin E
Database Links Reactome: R-HSA-6798695
Cellular Localisation Secreted
Lysosome
Endocytosed By Sort1 And Delivred To Lysosomes
Targeted To Lysosome By Psap Via M6pr And Lrp1
In Both Biosynthetic And Endocytic Pathways
Co-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome
Alternative ELISA Names Progranulin ELISA kit
Pgrn ELISA kit
Acrogranin ELISA kit
Epithelin Precursor ELISA kit
Glycoprotein Of 88 Kda ELISA kit
Gp88 ELISA kit
Glycoprotein 88 ELISA kit
Granulin Precursor ELISA kit
Pc Cell-Derived Growth Factor ELISA kit
Pcdgf ELISA kit
Proepithelin ELISA kit
Pepi Cleaved Into - Paragranulin - Granulin-1 ELISA kit
Granulin G - Granulin-2 ELISA kit
Granulin F - Granulin-3 ELISA kit
Epithelin-2 ELISA kit
Granulin B - Granulin-4 ELISA kit
Epithelin-1 ELISA kit
Granulin A - Granulin-5 ELISA kit
Granulin C - Granulin-6 ELISA kit
Granulin D - Granulin-7 ELISA kit
Granulin E ELISA kit
GRN ELISA kit
Specificity This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-111 102
EDTA plasma (n=8) 85-98 91
Cell culture media (n=8) 85-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 206.38 339.77 1769.00 224.98 330.28 1882.28
Standard deviation 11.74 15.73 83.14 12.82 18.99 64.37
CV (%) 5.69 4.63 4.70 5.70 5.75 3.42
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance