| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human GRB10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GRB10 |
| Gene ID: |
2887 |
| Uniprot ID: |
GRB10_HUMAN |
| Specificity: |
This kit recognizes Human Grb10 in samples. No significant cross-reactivity or interference between Human Grb10 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain. |
| Post Translational Modifications | Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation. Phosphorylated at Tyr-67 by TEC. |
| Function | Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. |
| Protein Name | Growth Factor Receptor-Bound Protein 10Grb10 Adapter ProteinInsulin Receptor-Binding Protein Grb-Ir |
| Database Links | Reactome: R-HSA-1433557Reactome: R-HSA-74713Reactome: R-HSA-74749Reactome: R-HSA-74751Reactome: R-HSA-8853659Reactome: R-HSA-9607240Reactome: R-HSA-9648895 |
| Cellular Localisation | CytoplasmWhen Complexed With Nedd4 And Igf1rFollows Igf1r InternalizationRemaining Associated With Early EndosomesUncouples From Igf1r-Containing Endosomes Before The Sorting Of The Receptor To The Lysosomal Compartment |
| Alternative CLIA Names | Growth Factor Receptor-Bound Protein 10 CLIA kitGrb10 Adapter Protein CLIA kitInsulin Receptor-Binding Protein Grb-Ir CLIA kitGRB10 CLIA kitGRBIR CLIA kitKIAA0207 CLIA kit |
| Specificity | This kit recognizes Human Grb10 in samples. No significant cross-reactivity or interference between Human Grb10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 98 |
| EDTA plasma(n=8) | 97-109 | 102 |
| Cell culture media(n=8) | 100-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 196.78 | 562.61 | 1937.24 | 196.12 | 605.91 | 1894.66 |
| Standard deviation | 20.86 | 57.05 | 153.43 | 18.12 | 50.41 | 126.37 |
| CV (%) | 10.60 | 10.14 | 7.92 | 9.24 | 8.32 | 6.67 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
