| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.061ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This GPX4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
GPX4 |
| Gene ID: |
2879 |
| Uniprot ID: |
GPX4_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Present primarily in testis. Expressed in platelets (at protein level). |
| Post Translational Modifications | |
| Function | Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins. Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide. Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis. The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures. May be required to protect cells from the toxicity of ingested lipid hydroperoxides. Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion. Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism. Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway. |
| Protein Name | Phospholipid Hydroperoxide Glutathione PeroxidasePhgpxGlutathione Peroxidase 4Gpx-4Gshpx-4 |
| Database Links | Reactome: R-HSA-2142688Reactome: R-HSA-2142712Reactome: R-HSA-2142770Reactome: R-HSA-9018676 P36969-2Reactome: R-HSA-9018896 P36969-2Reactome: R-HSA-9020265 P36969-2Reactome: R-HSA-9023661 P36969-2 |
| Cellular Localisation | Isoform Mitochondrial: MitochondrionIsoform Cytoplasmic: Cytoplasm |
| Alternative ELISA Names | Phospholipid Hydroperoxide Glutathione Peroxidase ELISA kitPhgpx ELISA kitGlutathione Peroxidase 4 ELISA kitGpx-4 ELISA kitGshpx-4 ELISA kitGPX4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
